B6;SJL-Tg(FCGR2A)11Mkz/J

Stock number: 003542
Product name: FcγRIIA
Research areas: Cancer Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research

Description

These FcγRIIA mice express a transgene expressing human FCGR2A on mouse platelets and macrophages, and exhibit a more severe antibody-mediated thrombocytopenia wild type mice.

Detailed description

Studies of the immune destruction of blood cells, such as platelets in immune thrombocytopenia, serve as a model for exploring the pathophysiology of these disorders. In general, mice lack the genetic equivalent of human FCGR2A, which is the FC receptor for IgG. This strain expresses human FCGR2A on mouse platelets and macrophages at levels equivalent to that observed in human cells. Antibody-mediated thrombocytopenia is significantly more severe than that observed in wild type mice. There is no phenotype in the absence of anti-platelet antibodies.

Allele

Symbol: Tg(FCGR2A)11Mkz
Name: transgene insertion 11, Steven E McKenzie
MGI accession ID: MGI:3056729
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Synonyms: FcgammaRIIA^TGN; huFCGR2A^R131
Marker symbol: Tg(FCGR2A)11Mkz
Marker name: transgene insertion 11, Steven E McKenzie
Chromosome: UN
Strain of origin: (C57BL/6 x SJL)F2
Expressed genes: FCGR2A,Fc fragment of IgG receptor IIa,human
Molecular note: The transgene contains the human FCGR2A gene. The genomic clone used in the transgene encodes the R¹³¹ polymorphic form of human FcgRIIA, which binds the widest range of mouse IgG subclasses including mouse IgG1 and rat IgG2a as well. 10 copies of the transgene was estimated to have inserted into the genome. Transgenic mice express human FCGR2A on mouse platelets and macrophages at levels equivalent to that observed in human cells.