These FcγRIIA mice express a transgene expressing human FCGR2A on mouse platelets and macrophages, and exhibit a more severe antibody-mediated thrombocytopenia wild type mice.
Dr. Steven E. McKenzie, Thomas Jefferson University
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
Studies of the immune destruction of blood cells, such as platelets in immune thrombocytopenia, serve as a model for exploring the pathophysiology of these disorders. In general, mice lack the genetic equivalent of human FCGR2A, which is the FC receptor for IgG. This strain expresses human FCGR2A on mouse platelets and macrophages at levels equivalent to that observed in human cells. Antibody-mediated thrombocytopenia is significantly more severe than that observed in wild type mice. There is no phenotype in the absence of anti-platelet antibodies.
Expressed Gene | FCGR2A, Fc fragment of IgG receptor IIa, human |
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Site of Expression |
Allele Name | transgene insertion 11, Steven E McKenzie |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | huFCGR2AR131 |
Gene Symbol and Name | Tg(FCGR2A)11Mkz, transgene insertion 11, Steven E McKenzie |
Gene Synonym(s) | |
Promoter | FCGR2A, Fc fragment of IgG receptor IIa, human |
Expressed Gene | FCGR2A, Fc fragment of IgG receptor IIa, human |
Strain of Origin | (C57BL/6 x SJL)F2 |
Chromosome | UN |
Molecular Note | The transgene contains the human FCGR2A gene. The genomic clone used in the transgene encodes the R131 polymorphic form of human FcgRIIA, which binds the widest range of mouse IgG subclasses including mouse IgG1 and rat IgG2a as well. 10 copies of the transgene was estimated to have inserted into the genome. Transgenic mice express human FCGR2A on mouse platelets and macrophages at levels equivalent to that observed in human cells. |
Mutations Made By | Dr. Michael Reilly, Thomas Jefferson University |
Homozygotes are viable and fertile. The expected coat colors are black, white-bellied agouti, and albino.
When using the FcγRIIA mouse strain in a publication, please cite the originating article(s) and include JAX stock #003542 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non carrier for Tg(FCGR2A)11Mkz |
Frozen Mouse Embryo | B6;SJL-Tg(FCGR2A)11Mkz/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;SJL-Tg(FCGR2A)11Mkz/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;SJL-Tg(FCGR2A)11Mkz/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;SJL-Tg(FCGR2A)11Mkz/J Frozen Embryo | $3373.50 |
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