These Hgs knock-out mice exhibit development with the ventral region outside of the yolk sac, two independent bilateral heart tubes, lack of a foregut, and die around embryonic day 11. They are suitable for use in applications related to the study of vesicular trafficking.
Dr. Philippe Soriano, Mount Sinai School of Medicine
Hgs homozygous mutant embryos develop with their ventral region outside of the yolk sac, have two independent bilateral heart tubes (cardia bifida), lack a foregut, and die around embryonic day 11 (E11.0). These phenotypes arise from a defect in ventral folding morphogenesis that occurs normally around E8.0. Significant apoptosis is detected in the ventral region of mutant embryos within the definitive endoderm, suggesting an important role for this germ layer in ventral folding morphogenesis. Abnormally enlarged early endosomes are detected in the mutants in several tissues including definitive endoderm, suggesting that a deficiency in vesicular transport via early endosomes underlies the mutant phenotype. The vesicular localization of Hgs is disrupted in cells treated with wortmannin, implicating Hgs in the phosphatidylinositol 3-kinase pathway of membrane trafficking.
|Allele Name||targeted mutation 1, Philippe Soriano|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Hgs, HGF-regulated tyrosine kinase substrate|
|Strain of Origin||129S4/SvJaeSor|
|Molecular Note||A neomycin resistance cassette replaced a 2.1 kb segment of the gene, which included exons 2, 3, and 4, encompassing the amino terminal region of the protein. In addition, the insertion produced a frameshift of exons 1 and 5.|
|Mutations Made By|| |
Dr. Philippe Soriano, Mount Sinai School of Medicine
When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to C57BL/6J inbred mice (Stock No. 000664). Homozygous mice are die by E11.
When using the Hrs- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003539 in your Materials and Methods section.
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