These Hgs knock-out mice exhibit development with the ventral region outside of the yolk sac, two independent bilateral heart tubes, lack of a foregut, and die around embryonic day 11. They are suitable for use in applications related to the study of vesicular trafficking.
Dr. Philippe Soriano, Mount Sinai School of Medicine
Genetic Background | Generation |
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000664 C57BL/6J |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Hgs | HGF-regulated tyrosine kinase substrate |
Hgs homozygous mutant embryos develop with their ventral region outside of the yolk sac, have two independent bilateral heart tubes (cardia bifida), lack a foregut, and die around embryonic day 11 (E11.0). These phenotypes arise from a defect in ventral folding morphogenesis that occurs normally around E8.0. Significant apoptosis is detected in the ventral region of mutant embryos within the definitive endoderm, suggesting an important role for this germ layer in ventral folding morphogenesis. Abnormally enlarged early endosomes are detected in the mutants in several tissues including definitive endoderm, suggesting that a deficiency in vesicular transport via early endosomes underlies the mutant phenotype. The vesicular localization of Hgs is disrupted in cells treated with wortmannin, implicating Hgs in the phosphatidylinositol 3-kinase pathway of membrane trafficking.
Allele Name | targeted mutation 1, Philippe Soriano |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Hrs- |
Gene Symbol and Name | Hgs, HGF-regulated tyrosine kinase substrate |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJaeSor |
Chromosome | 11 |
Molecular Note | A neomycin resistance cassette replaced a 2.1 kb segment of the gene, which included exons 2, 3, and 4, encompassing the amino terminal region of the protein. In addition, the insertion produced a frameshift of exons 1 and 5. |
Mutations Made By | Dr. Philippe Soriano, Mount Sinai School of Medicine |
When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to C57BL/6J inbred mice (Stock No. 000664). Homozygous mice are die by E11.
When using the Hrs- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003539 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wild-type for Hgs<tm1Sor> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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