STOCK Tg(GFAP-TVA)5Hev/J

Stock number: 003528
Product name: Gtv-a
Research areas: Research Tools

Description

These Gtv-a mice express a transgene expressing the avian cell surface receptor for subgroup A avian leukosis viruses directed by an astrocyte-specific promoter derived from the human glial fibrillary acidic protein gene.

Detailed description

This strain expresses the avian cell surface receptor(TVA) for subgroup A avian leukosis viruses. The transgene is under the control of an astrocyte-specific promoter derived from the human glial fibrillary acidic protein (GFAP ) gene. The result is to render glial cells specifically susceptible to infection by viral vectors making possible the glia-specific viral transfer of genes.

Development

The transgenic construct consists of the GFAP promoter, TVA gene (avian cell surface receptor)and a portion of the mouse protamine (Prm1) gene which supplies intronic sequence and a polyadenylation site. The strain was created on a FVB/N background and was then crossed with mice on a B6;129 background.

Allele

Symbol: Tg(GFAP-TVA)5Hev
Name: transgene insertion 5, Harold E Varmus
MGI accession ID: MGI:2663943
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(GFAP-TVA)5Hev
Marker name: transgene insertion 5, Harold E Varmus
Chromosome: UN
Strain of origin: mixed
Expressed genes: TVA,subgroup A avian leukosis virus receptor,quail
Molecular note: The transgene consists of the astrocyte-specific human glial fibrillary acidic protein (GFAP) promoter, TVA gene (avian cell surface receptor), and a portion of the mouse protamine (Prm1) gene which supplies intronic sequence and a polyadenylation site. The transgene expresses the quail TVA for subgroup A avian leukosis viruses.
General note: The construct was injected into embryos generated by crossing an FVB/N mouse to a (C57BL/6 x BALB/c)F1 mouse. Glial cells in transgenic mice are specifically susceptible to infection by replication-competent ALV splice acceptor (RCAS) retroviral vectors.