These Gtv-a mice express a transgene expressing the avian cell surface receptor for subgroup A avian leukosis viruses directed by an astrocyte-specific promoter derived from the human glial fibrillary acidic protein gene.
Dr. Wendy Hively, NIH - NCI
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
This strain expresses the avian cell surface receptor(TVA) for subgroup A avian leukosis viruses. The transgene is under the control of an astrocyte-specific promoter derived from the human glial fibrillary acidic protein (GFAP ) gene. The result is to render glial cells specifically susceptible to infection by viral vectors making possible the glia-specific viral transfer of genes.
The transgenic construct consists of the GFAP promoter, TVA gene (avian cell surface receptor)and a portion of the mouse protamine (Prm1) gene which supplies intronic sequence and a polyadenylation site. The strain was created on a FVB/N background and was then crossed with mice on a B6;129 background.
Expressed Gene | TVA, subgroup A avian leukosis virus receptor, quail |
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Site of Expression |
Allele Name | transgene insertion 5, Harold E Varmus |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | Gtv-a |
Gene Symbol and Name | Tg(GFAP-TVA)5Hev, transgene insertion 5, Harold E Varmus |
Gene Synonym(s) | |
Promoter | GFAP, glial fibrillary acidic protein, human |
Expressed Gene | TVA, subgroup A avian leukosis virus receptor, quail |
Strain of Origin | mixed |
Chromosome | UN |
General Note | The construct was injected into embryos generated by crossing an FVB/N mouse to a (C57BL/6 x BALB/c)F1 mouse. Glial cells in transgenic mice are specifically susceptible to infection by replication-competent ALV splice acceptor (RCAS) retroviral vectors. |
Molecular Note | The transgene consists of the astrocyte-specific human glial fibrillary acidic protein (GFAP) promoter, TVA gene (avian cell surface receptor), and a portion of the mouse protamine (Prm1) gene which supplies intronic sequence and a polyadenylation site. The transgene expresses the quail TVA for subgroup A avian leukosis viruses. |
Mutations Made By | Dr. Wendy Hively, NIH - NCI |
When maintaining a live colony, hemizygous mice may be bred to wildtype mice from the colony.
When using the Gtv-a mouse strain in a publication, please cite the originating article(s) and include JAX stock #003528 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizyous or Non Carrir for Tg(GFAP-TVA)5Hev |
Frozen Mouse Embryo | STOCK Tg(GFAP-TVA)5Hev/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(GFAP-TVA)5Hev/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(GFAP-TVA)5Hev/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(GFAP-TVA)5Hev/J Frozen Embryo | $3373.50 |
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