These Blmh knock-out mice exhibit variably penetrant tail dermatitis that resembles rodent ringtail, resembling skin lesions in humans with pellagra, necrolytic migratory erythema, and acrodermatitis enteropathica.
Dr. John S. Lazo, University of Pittsburgh
Bleomycin (BLM) is a clinically used glycopeptide anticancer agent. It is deaminated in vitro by BLMH. Blmh null mice have decreased viability and fertitility. Only about 65% of the expected number survive the neonatal period. Mice lacking Blmh exhibit variably penetrant tail dermatitis that resembles rodent ringtail. This resembles skin lesions in humans with pellagra, necrolytic migratory erythema, and acrodermatitis enteropathica. Null mice also are more sensitive to acute BLM lethality and develop pulmonary fibrosis following BLM treatment.
|Allele Name||targeted mutation 1, Gregg E Homanics|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Bh-; Blmhtm2Geh|
|Gene Symbol and Name||Blmh, bleomycin hydrolase|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A genomic fragment containing exon 3 and flanking DNA was replaced with a neomycin selection cassette. The deleted sequences include the region encoding the catalytic cysteine of the protein. Northern blot analysis on total embryonic RNA derived from homozygous mice demonstrated that no transcript was produced from this allele. Western blot and enzymatic activity assays confirmed that no functional protein was made in homozygous mice.|
|Mutations Made By|| |
Donald Schwartz, Wayne State Univ School of Medicine
When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to C57BL/6J inbred mice (Stock No. 000664). Although viable and fertile, the donating investigator finds heterozygotes are more productive than homozygotes. Do not disturb on the day of birth or they will canabalize their litters. Most newborn pups exhibit unusual dermatopathy which resolves at about 7-10 days. Gross tail lesions persist on the null mice, regardless of genetic background. This latter condition is exacerbated by low relative humidity in the mouse room.
When using the Bh- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003509 in your Materials and Methods section.