Mice carrying the spontaneous mutant Nox3het-2J exhibit head-leaning and circling; they may be useful in studies relating to the formation of otoconia.
Read More +Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | Nox3 | NADPH oxidase 3 |
Marker Symbol | Marker Name | |
---|---|---|
H2 | histocompatibility-2, MHC |
Mice with the head tilt (het, now Nox3het) mutation are characterized by impaired otoconial morphogenesis with normal functionality of the auditory system. Mice carrying the spontaneous mutant Nox3het-2J exhibit head-leaning and circling, which are more dramatic than the original Nox3het mutation. However, auditory-evoked brainstem response testing of three 5-week old homozygotes and heterozygotes showed normal hearing indistinguishable from wild-type controls and the original het homozygotes. Viability and fertility of both sexes of mice are normal, although trampling of pups is common.
The mutation in the het-2J mouse was identified as a G to A substitution at nucleotide position 1282, replacing a glycine residue with an arginine a residue. B10.Q-H2q/SgJ-het2J/+ mice were crossed to C57BL/6J for cryopreservation.
Allele Name | head tilt 2 Jackson |
---|---|
Allele Type | Spontaneous |
Allele Synonym(s) | |
Gene Symbol and Name | Nox3, NADPH oxidase 3 |
Gene Synonym(s) | |
Strain of Origin | B10.Q-H2q/SgJ |
Chromosome | 17 |
General Note | The head-leaning and circling are more dramatic than the original het mutation. However, ABR (auditory-evoked brainstem response) testing of three 5-week old homozygotes and heterozygotes showed normal hearing indistinquishable from wild-type controls and the original het/het mutants. Viability and fertility of both sexes of mice are normal; although trampling of pups is common(J:56803). |
Molecular Note | The mutation in the het-2J mouse was identified as a G-to-A substitution at coding nucleotide position 1222 (c.1222G>A), replacing a glycine residue with an arginine at position 408 (p.G408R). The glycine residue is highly conserved and is part of an NADPH binding site. |
Marker Synonym(s) | |
---|---|
Chromosome(s) | 17 |
/SgJ
When using the head tilt 2 Jackson mouse strain in a publication, please cite the originating article(s) and include JAX stock #003483 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Nox3<het-2J> |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.