This strain was made using gene targeting in embryonic stem cells to modify the Il4 locus by knocking-in a transmembrane domain. Expression of Il4 in a membrane-bound form provides a novel method for the identification and characterization of Il4-producing cells.
|Allele Name||targeted mutation 1, Michael Grusby|
|Allele Type||Targeted (Inserted expressed sequence)|
|Gene Symbol and Name||Il4, interleukin 4|
|Promoter||Il4, interleukin 4, mouse, laboratory|
|Strain of Origin||129S2/SvPas|
|General Note||This mutation resulted in mice that express Il4 in a membrane-bound form for use in identifying and characterizing Il4-producing cells.|
|Molecular Note||A modified version of exon 4 of the gene was inserted between the last codon and the translational stop site of the gene. This modified version of exon 4 contained a transmembrane anchor. In addition, a neomycin resistance cassette was inserted 150 nucleotides downstream of the polyadenylation signal of the gene. These modifications allowed production of mice that express a membrane-bound form of the protein.|
|Mutations Made By|| |
Dr. Michael Grusby, Harvard Medical School
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