C57BL/6J-Tg(SV)419Bri/J

Stock number: 003477
Product name: 419-line
Research areas: Cancer Research

Description

These 419-line mice express a transgene containing SV40 T antigen early region genes. They show a low incidence of brain choroid plexus tumors and a normal immune response to SV40 T antigen.

Detailed description

This 419 transgenic strain and the 427 strain C57BL/6J-Tg(SV)427Bri/J (Stock No. 003476) each contain the same SV40 T antigen early region genes in the SVMK plasmid construct, but differ in choroid plexus tumor incidence and immune response to SV40 T antigen. Strain 419 mice developed 1 tumor out of 53 mice, while strain 427 mice developed 95 tumors out of 96 mice. Strain 419 mice mount a normal immune response to SV40 T antigen compared with non-transgenic control mice while strain 427 mice are completely unresponsive to immunization with SV40. (Faas SJ, Pan S, Pinkert CA, Brinster RL, Knowles BB. Simian virus 40 (SV40)-transgenic mice that develop tumors are specifically tolerant to SV40 T antigen. J Exp Med. 1987 Feb 1;165(2):417-27.)

Development

The strain was generated on a C57BL/6J background and is currently on the same background. It carries the SVMK plasmid consisting of a pSV3-ts58-derived Kpn I-Bam HI fragment.

Allele

Symbol: Tg(SV)419Bri
Name: transgene insertion 419, Ralph L Brinster
MGI accession ID: MGI:3056380
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(SV)419Bri
Marker name: transgene insertion 419, Ralph L Brinster
Chromosome: UN
Strain of origin: C57BL/6J
Expressed genes: TAg,SV40 large T-antigen,SV40
Molecular note: The transgene contains the SV40 enhancer and large-T antigen.