Homozygous mice carrying the Tg(HLA-A2.1)1Enge transgene express significant quantities of the human class I MHC Ag HLA-A2.1 on cells from the spleen, bone marrow and thymus. These transgenic mice have been used to identify hepatitic C virus (HCV) peptides expressing a sequence for HLA-A2.1 binding that are actually recognized by human A2.1-restricted CTLs. Thus, this transgenic model is important for the study of HLA-restricted CTL determinants and in potential development of a vaccine against HCV.
Dr. Victor H. Engelhard, University of Virginia
Genetic Background | Generation |
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Allele Type |
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Transgenic (Null/Knockout, Inserted expressed sequence, Humanized sequence) |
Homozygous mice carrying the Tg(HLA-A2.1)1Enge transgene express significant quantities of the human class I MHC Ag HLA-A2.1 on cells from the spleen, bone marrow and thymus. The transgene integrated into chromosome 8 causing a duplication in Mcph1 (microcephaly, primary autosomal recessive 1). The duplication results in a functional knock-out of Mcph1 in homozygous mice. Expression of this human class I molecule did not result in expansion of the number of cytotoxic T lymphocyte (CTL) precursors specific for other human class I Ag, HLA-B27 or HLA-A2.2. These transgenic mice have been used to identify hepatitic C virus (HCV) peptides expressing a sequence for HLA-A2.1 binding that are actually recognized by human A2.1-restricted CTLs. Thus, this transgenic model is important for the study of HLA-restricted CTL determinants and in potential development of a vaccine against HCV.
In 2018, protein expression was verified by flow cytometry in male and female splenocytes using an anti-HLA-A2 antibody (Clone CR11-351).
Transgenic mice were constructed by microinjection of the 7-kb EcoRI fragment containing the full length HLA-A2.1 gene into fertilized eggs from C57BL/6 mice. The Tg(HLA-A2.1)1Enge transgene integrated into chromosome 8 causing a duplication in Mcph1 (microcephaly, primary autosomal recessive 1).
Expressed Gene | HLA-A, major histocompatibility complex, class I, A, human |
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Site of Expression |
Allele Name | transgene insertion 1, Victor H Engelhard |
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Allele Type | Transgenic (Null/Knockout, Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Mcph1, microcephaly, primary autosomal recessive 1 |
Gene Synonym(s) | |
Promoter | HLA-A2.1, major histocompatibility complex, class I, subtype A2.1, human |
Expressed Gene | HLA-A, major histocompatibility complex, class I, A, human |
Strain of Origin | C57BL/6 |
Chromosome | 8 |
General Note | Expression of this human class I molecule in transgenic mice does not result in expansion of the number of cytotoxic T lymphocyte (CTL) precursors specific for other human class I Ag, HLA-B27 or HLA-A2.2. |
Molecular Note | The transgene contains the full length HLA-A2.1 gene. Expression of human class I MHC Ag HLA-A2.1 was detected on cells from homozygous transgenic spleen, bone marrow, and thymus. Line 1 inserted into the gene at 18736683-18757058 (Build GRCm38/mm10) resulting in a duplication. The duplication results in a functional knock-out. Founder line 1 has a copy number of 3-8. |
Mutations Made By | Dr. Ai-Xuan Le, Stanford University |
When using the HLA-A2 mouse strain in a publication, please cite the originating article(s) and include JAX stock #003475 in your Materials and Methods section.
Service/Product | Description | Price |
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Hemizygous for Tg(HLA-A2.1)1Enge |
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