Overview

Also Known As: HLA-A2

Homozygous mice carrying the Tg(HLA-A2.1)1Enge transgene express significant quantities of the human class I MHC Ag HLA-A2.1 on cells from the spleen, bone marrow and thymus. These transgenic mice have been used to identify hepatitic C virus (HCV) peptides expressing a sequence for HLA-A2.1 binding that are actually recognized by human A2.1-restricted CTLs. Thus, this transgenic model is important for the study of HLA-restricted CTL determinants and in potential development of a vaccine against HCV.

Donating Investigator

Dr. Victor H. Engelhard, University of Virginia

Genetic overview

Genetic Background	Generation

Mcph1^{Tg(HLA-A2.1)1Enge}

Allele Type
Transgenic (Null/Knockout, Inserted expressed sequence, Humanized sequence)

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Research Applications

Immunology, Inflammation and Autoimmunity Research

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Base Price

Starting at:

$139.38 Domestic price for female 3-week

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Details

Detailed Description

Homozygous mice carrying the Tg(HLA-A2.1)1Enge transgene express significant quantities of the human class I MHC Ag HLA-A2.1 on cells from the spleen, bone marrow and thymus. The transgene integrated into chromosome 8 causing a duplication in Mcph1 (microcephaly, primary autosomal recessive 1). The duplication results in a functional knock-out of Mcph1 in homozygous mice. Expression of this human class I molecule did not result in expansion of the number of cytotoxic T lymphocyte (CTL) precursors specific for other human class I Ag, HLA-B27 or HLA-A2.2. These transgenic mice have been used to identify hepatitic C virus (HCV) peptides expressing a sequence for HLA-A2.1 binding that are actually recognized by human A2.1-restricted CTLs. Thus, this transgenic model is important for the study of HLA-restricted CTL determinants and in potential development of a vaccine against HCV.

In 2018, protein expression was verified by flow cytometry in male and female splenocytes using an anti-HLA-A2 antibody (Clone CR11-351).

Development

Transgenic mice were constructed by microinjection of the 7-kb EcoRI fragment containing the full length HLA-A2.1 gene into fertilized eggs from C57BL/6 mice. The Tg(HLA-A2.1)1Enge transgene integrated into chromosome 8 causing a duplication in Mcph1 (microcephaly, primary autosomal recessive 1).

Expression Data

Expressed Gene	HLA-A, major histocompatibility complex, class I, A, human
Site of Expression

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Le AX; Bernhard EJ; Holterman MJ; Strub S; Parham P; Lacy E; Engelhard VH. 1989. Cytotoxic T cell responses in HLA-A2.1 transgenic mice. Recognition of HLA alloantigens and utilization of HLA-A2.1 as a restriction element. J Immunol 142(4):1366-71PubMed: 2464645 MGI: J:52612

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Genetics

Mcph1^{Tg(HLA-A2.1)1Enge}

Allele Symbol: Mcph1^{Tg(HLA-A2.1)1Enge}

Allele Name	transgene insertion 1, Victor H Engelhard
Allele Type	Transgenic (Null/Knockout, Inserted expressed sequence, Humanized sequence)
Allele Synonym(s)	transgene insertion 1, Victor H Engelhard; Mcph1^{Tg(HLA-A2.1)1Enge}
Gene Symbol and Name	Mcph1, microcephaly, primary autosomal recessive 1
Gene Synonym(s)	5430437K10Rik; D030046N04Rik; BRIT1; 5430437K10Rik; RGD1560642; RIKEN cDNA 5430437K10 gene; MCT; D030046N04Rik; RIKEN cDNA D030046N04 gene
Promoter	HLA-A2.1, major histocompatibility complex, class I, subtype A2.1, human
Expressed Gene	HLA-A, major histocompatibility complex, class I, A, human
Strain of Origin	C57BL/6
Chromosome	8
General Note	Expression of this human class I molecule in transgenic mice does not result in expansion of the number of cytotoxic T lymphocyte (CTL) precursors specific for other human class I Ag, HLA-B27 or HLA-A2.2.
Molecular Note	The transgene contains the full length HLA-A2.1 gene. Expression of human class I MHC Ag HLA-A2.1 was detected on cells from homozygous transgenic spleen, bone marrow, and thymus. Line 1 inserted into the gene at 18736683-18757058 (Build GRCm38/mm10) resulting in a duplication. The duplication results in a functional knock-out. Founder line 1 has a copy number of 3-8.
Mutations Made By	Dr. Ai-Xuan Le, Stanford University

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
- HLA transgenics

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Mcph1^{Tg(HLA-A2.1)1Enge}/Mcph1⁺
involves: C57BL/6

immune system phenotype

abnormal immune tolerance
- transgenic animals are more tolerant to HLA-A2.1-expressing murine cells than control mice primed once with influenza virus; numbers of cytotoxic T lymphocyte precursors are 100-fold higher in primed C57BL/6 controls

References

Le AX; Bernhard EJ; Holterman MJ; Strub S; Parham P; Lacy E; Engelhard VH. 1989. Cytotoxic T cell responses in HLA-A2.1 transgenic mice. Recognition of HLA alloantigens and utilization of HLA-A2.1 as a restriction element. J Immunol 142(4):1366-71PubMed: 2464645 MGI: J:52612

Additional References

Newberg MH; Smith DH; Haertel SB; Vining DR; Lacy E; Engelhard VH. 1996. Importance of MHC class 1 alpha2 and alpha3 domains in the recognition of self and non-self MHC molecules. J Immunol 156(7):2473-80PubMed: 8786307 MGI: J:31937

Additional - Mcph1^{Tg(HLA-A2.1)1Enge} related

Technical Support

Contact Technical Support

Genotyping Protocols
- QPCR:Tg(HLA-A)-2
- QPCR:Tg(HLA-A2.1)1Enge-qPCR
- Standard PCR:Tg(HLA-A2.1)1Enge
- Genotyping resources and troubleshooting
Breeding Considerations

This strain is a good breeder.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Homozygote
Appearance
- black
  Related Genotype: a/a
Citation
When using the HLA-A2 mouse strain in a publication, please cite the originating article(s) and include JAX stock #003475 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX4 (Standard)

Pricing & Availability

Available Now

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Hemizygous for Tg(HLA-A2.1)1Enge

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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Also Known As: HLA-A2

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Mcph1Tg(HLA-A2.1)1Enge

Allele Symbol: Mcph1Tg(HLA-A2.1)1Enge

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Mcph1Tg(HLA-A2.1)1Enge/Mcph1+involves: C57BL/6

immune system phenotype

References

Additional References

Additional - Mcph1Tg(HLA-A2.1)1Enge related

Genotyping Protocols

Breeding Considerations

Mating System

Appearance

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Mcph1^{Tg(HLA-A2.1)1Enge}

Allele Symbol: Mcph1^{Tg(HLA-A2.1)1Enge}

Genotype: Mcph1^{Tg(HLA-A2.1)1Enge}/Mcph1⁺
involves: C57BL/6

Additional - Mcph1^{Tg(HLA-A2.1)1Enge} related