These Gt(ROSA)26Sortm1Sor targeted mutant mice carry a loxP flanked neo cassette upstream of a β-galactosidase (lacZ) sequence. Removal of the neo cassette by cre recombination results in lacZ expression in cre-expressing tissues of the offspring. These mutant mice may be used as a Cre-reporter strain to test the tissue/cellular expression pattern of cre expressing mice.
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
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000664 C57BL/6J |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), Reporter) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
Homozygous mice are viable and fertile, with a loxP-flanked DNA STOP sequence preventing expression of the downstream lacZ gene. When crossed with a cre transgenic strain, the STOP sequence is removed and lacZ is expressed in cells/tissues where cre is expressed. These mutant mice may be used as a Cre-reporter strain; to test the tissue/cellular expression pattern of cre transgenic mice.
The R26R targeting construct was created by subcloning the pROSA26-1 vector, and inserting a splice acceptor sequence (identical to the one used in the original gene trap allele), a loxP-flanked STOP sequence (containing a neo cassette and triple polyadenylation (STOP) sequence), a lacZ gene, and a polyadenylation sequence at a unique site approximately 300-bp 5' of the original gene trap integration site. Transcriptional read-through is prevented with the use of a triple polyadenylation (STOP) sequence at the 3' end of the neo expression cassette. The construct was electroporated into 129S4/SvJaeSor derived AK7 embryonic stem (ES) cells. This strain arose on a B6;129 background and was backcrossed to C57BL/6J utilizing a marker-assisted protocol.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | when crossed to a Cre recombinase-expressing strain, lacZ expression is observed in the cre-expressing tissues |
Allele Name | targeted mutation 1, Philippe Soriano |
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Allele Type | Targeted (Conditional ready (e.g. floxed), Reporter) |
Allele Synonym(s) | Gt(ROSA)26Sor; Gt(ROSA)26Sortm1(LacZ)Cos; Gtrosa26tm/Sor; Gtrosa26tm1Sor; loxP-Stop-loxP lacZ reporter; LSL-LacZ; R26fslz; R26LacZ; R26-Cond-lacZ; R26-floxstop-lacZ; R26-LNL-LacZ; R26R; R26RLacZ; R26RZ; R26R-lacZ; RosaLoxP; Rosa26 cre reporter; ROSA26::LNL::LacZ; ROSA26fl; Rosa26LacZ; Rosa26stop-LacZ; ROSA26-floxed Neo-LacZ; ROSA26-flox-STOP-flox-lacZ; ROSA26-LacZ; Rosa26-lacZ cre reporter; Rosa26-LacZloxP; ROSA26-loxP; Rosa26loxP-STOP-lacZ; Rosa26-LoxSTOPLox-LacZ; ROSA26R; Rosa26r-; ROSA26Rflox; Rosa26RLacZ; Rosa26RlacZ; Rosa26R-lacZ; ROSA26Sortm1Sor; ROSA26XneorXlacZ; Rosa2R; Rosa-LoxP-Stop-LoxP-LacZ; RS; STOP-lacZ |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | when crossed to a Cre recombinase-expressing strain, lacZ expression is observed in the cre-expressing tissues |
Strain of Origin | 129S4/SvJaeSor |
Chromosome | 6 |
Molecular Note | A targeting vector was designed from the original gene trap strain, ROSA beta-geo26, to include a splice acceptor sequence (SA), a neo expression cassette flanked by loxP sites, a lacZ gene, and a polyadenylation (bpA) sequence inserted at a unique Xba1 site approximately 300-bp 5' of the original gene-trap integration site. In addition, a triple polyadenylation sequence was added to the 3' end of the neo expression cassette to prevent transcriptional read-through. Presence of the floxed neo cassette prevents lacZ expression. When crossed with a cre transgenic strain, lacZ is expressed in all cells/tissue where Cre is expressed. |
Mutations Made By | Dr. Philippe Soriano, Mount Sinai School of Medicine |
Homozygous mice are viable and fertile. When maintaining a live colony, homozygous mice may be bred together. Coat color expected from breeding is Black.
When using the R26R mouse strain in a publication, please cite the originating article(s) and include JAX stock #003474 in your Materials and Methods section.
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Heterozygous for Gt(ROSA)26Sor<tm1Sor> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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