These transgenic 354-line mice express the SV40 tumor antigen under control of the liver promoter of the mouse alpha-amylase gene (Amy1a), and develop malignant tumors of the brown adipose tissue.
Read More +Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
C57BL/6-TgN(Amy1TAg)354Knw mice are viable and fertile. They express the SV40 tumor antigen under control of the liver promoter of the mouse alpha-amylase gene (Amy-1a). This transgenic strain develops malignant tumors of the brown adipose tissue at about 18 months of age. The tumors have high metastatic potential, with metastases commonly occurring in liver, lung, spleen, heart and adrenal glands. Note: This line, '354', contains the same trangene construct as line '501' (Stock No. 003446). Divergent tumor development between these lines is reported to be a result of different transgene integration sites.
Expressed Gene | TAg, SV40 large T-antigen, SV40 |
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Site of Expression |
Allele Name | transgene insertion 354, Barbara Knowles |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | 354 |
Gene Symbol and Name | Tg(Amy1TAg)354Knw, transgene insertion 354, Barbara Knowles |
Gene Synonym(s) | |
Promoter | Amy1, amylase 1, salivary, mouse, laboratory |
Expressed Gene | TAg, SV40 large T-antigen, SV40 |
Strain of Origin | C57BL/6J |
Chromosome | UN |
Molecular Note | The transgene encodes an amylase 1 (salivary)-SV40 T-antigen hybrid protein and is expressed in the liver. Transgene expression is controlled by the mouse amylase 1, salivary, gene (Amy1) liver isoform promoter. Line 501 was also produced. |
Homozygotes are viable and fertile. When maintaining a live colony, transgene carrier mice may be bred together, to wildtype (non-carrier) mice from the colony or to C57BL/6J inbred mice (Stock No. 000664). Alternatively, homozygous mice may be bred together.
When using the 354-line mouse strain in a publication, please cite the originating article(s) and include JAX stock #003445 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Homozygous for Tg(Amy1TAg)354Knw |
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