C57BL/6J-Tg(Amy1TAg)354Knw/J

Stock number: 003445
Product name: 354-line
Research areas: Cancer Research

Description

These transgenic 354-line mice express the SV40 tumor antigen under control of the liver promoter of the mouse alpha-amylase gene (Amy1^a), and develop malignant tumors of the brown adipose tissue.

Detailed description

C57BL/6-TgN(Amy1TAg)354Knw mice are viable and fertile. They express the SV40 tumor antigen under control of the liver promoter of the mouse alpha-amylase gene (Amy-1^a). This transgenic strain develops malignant tumors of the brown adipose tissue at about 18 months of age. The tumors have high metastatic potential, with metastases commonly occurring in liver, lung, spleen, heart and adrenal glands. Note: This line, '354', contains the same trangene construct as line '501' (Stock No. 003446). Divergent tumor development between these lines is reported to be a result of different transgene integration sites.

Allele

Symbol: Tg(Amy1TAg)354Knw
Name: transgene insertion 354, Barbara Knowles
MGI accession ID: MGI:3056366
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Amy1TAg)354Knw
Marker name: transgene insertion 354, Barbara Knowles
Chromosome: UN
Strain of origin: C57BL/6J
Expressed genes: TAg,SV40 large T-antigen,SV40
Molecular note: The transgene encodes an amylase 1 (salivary)-SV40 T-antigen hybrid protein and is expressed in the liver. Transgene expression is controlled by the mouse amylase 1, salivary, gene (Amy1) liver isoform promoter. Line 501 was also produced.