C57BL/6J-Tg(HLA-B27)30-4Trg/DcrJ

Stock number: 003440
Product name: HLA-B27
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

These B27mice contain a transgene expressing the human HLA-B27 gene on lymphoid cells at levels comparable to endogenous H-2b and H-2s class I MHC molecules.

Detailed description

The H chain of the human class I MHC Ag HLA-B27 was introduced into the germ lines of inbred C57BL/6 (B6) mice by micro-injection resulting in physiologic cell surface expression of the HLAA B27 gene product. Function of the B27 Ag as the stimulator of both primary and secondary allogeneic cytolytic T cell responses demonstrated that the human transgene product functions as a normal class I MHC molecule in the mouse. These transgenic mice may be useful in studies of HLA-B27 in inflammatory human disease.

Development

The Tg(HLA-B27)30-4Trg allele was generated by injection into the male pronucleus of C57BL/6J fertilized eggs (Taurog et al., 1988).

Allele

Symbol: Tg(HLA-B27)30-4Trg
Name: transgene insertion 30-4, Joel D Taurog
MGI accession ID: MGI:2159095
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(HLA-B27)30-4Trg
Marker name: transgene insertion 30-4, Joel D Taurog
Chromosome: UN
Strain of origin: C57BL/6J
Expressed genes: HLA-B,major histocompatibility complex, class I, B,human
Molecular note: A transgene containing the human HLA-B allele, HLA-B27, was shown to be expressed in spleen cells of mice carrying the transgene.