These line 56-3 mice express a transgene containing the human B2M and HLA-B2705 sequences with high expression of HLA-B27.
Read More +Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
This transgenic strain carries the human B2M and HLA-B27:05 sequences. Transgenic constructs were co-injected and co-integrated; and founder line designated 56-3. Cell surface expression of B27 is several fold higher than mice expressing B27 alone. This transgenic insertion event is homozygous lethal.
The HLA-B2705 and human B2M constructs were co-injected into fertilized (C57BL/6 x SJL/J)F2 eggs by Dr. Joel Taurog (University of Texas, Southwestern) and co-integrated onto the same chromosome. The founder line was designated 56-3. Mice were backcrossed to C57BL/6 mice for at least 5 generations.
Expressed Gene | HLA-B, major histocompatibility complex, class I, B, human |
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Expressed Gene | B2M, beta-2-microglobulin, human |
Site of Expression |
Allele Name | transgene insertion 56-3, Joel D Taurog |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | line 56-3; Tg(B2M)1Trg; Tg(B2M)1Trg Tg(HLA-B27)1Trg; Tg(HLA-B)1Trg; Tg(HLA-B27)1Trg |
Gene Symbol and Name | Tg(B2M,HLA-B*27:05)56-3Trg, transgene insertion 56-3, Joel D Taurog |
Gene Synonym(s) | |
Promoter | HLA-B, major histocompatibility complex, class I, B, human |
Promoter | B2M, beta-2-microglobulin, human |
Expressed Gene | HLA-B, major histocompatibility complex, class I, B, human |
Expressed Gene | B2M, beta-2-microglobulin, human |
Strain of Origin | (C57BL/6 x SJL/J)F2 |
Chromosome | UN |
General Note | Because separate constructs were injected this transgenic insertion was called by two separate marker sybmols for many years, Tg(B2M)1Trg and Tg(HLA-B27)1Trg, but this was corrected in 2016 to a single marker indicative of probable co-integration. |
Molecular Note | The HLA-B*27:05 and human B2M constructs were co-injected and are believed to have co-integrated because they have co-segregated through extensive breeding. Splenic expression of both was confirmed. This insertion is believed to be homozygous lethal. |
This transgenic insertion event is homozygous lethal.
When using the line 56-3 mouse strain in a publication, please cite the originating article(s) and include JAX stock #003428 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(B2M)1Trg, Hemizygous or Non carrier for Tg(HLA-B)1Trg |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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