These β-actin-cre transgenic mice express Cre recombinase directed by the human beta actin gene promoter. Cre recombinase activity is widespread in the embryo by the blastocyst stage of development.
Dr. Gail Martin, University of California San Francisco
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
These β-actin-cre transgenic mice express Cre recombinase under the control of the human beta actin gene promoter. Cre recombinase activity is widespread in the embryo by the blastocyst stage of development. It is useful as a Cre-deleter mouse for virtually any genomic sequence flanked by loxP sites. Homozygotes are viable and fertile.
Of note, this allele is also available on a congenic C57BL/6J genetic background as Stock No. 033984 and on a congenic C57BL/6NJ background as Stock No. 019099.
A transgenic insert designed by Drs. Mark Lewandoski and Gail R. Martin (University of California, San Francisco) containing Cre recombinase under the control of the human beta-actin promoter was injected into fertilized FVB/N mouse eggs. Founder line 2, containing a single copy of the transgene inserted into intron 4 of transmembrane protein 163 (Tmem163) gene, was subsequently established. The mice were maintained on the FVB/N background.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | all cells of the embryo by the blastocyst stage of development; widespread expression |
Allele Name | transgene insertion 2, Gail R Martin |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | A-cre; ACTB-cre; Act-Cre; actin-cre; beta-actin::Cre; beta-actin:Cre; beta-actin-Cre; creactin; TgN(ACTB-cre)2Mrt |
Gene Symbol and Name | Tmem163, transmembrane protein 163 |
Gene Synonym(s) | |
Promoter | ACTB, actin beta, human |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | all cells of the embryo by the blastocyst stage of development; widespread expression |
Strain of Origin | FVB/N |
Chromosome | 1 |
Molecular Note | This transgene expresses Cre recombinase under the control of a human beta-actin promoter, which is active in all embryonic cells at or before the blastocyst stage. Line number 2 contains a single copy of the transgene inserted into intron 4 of Tmem163 (chr1:129,442,346; NCBI37/mm9) with three intronic deletions of 2.2, 7.3 and 3.2 kb, respectively, surrounding the transgene integration site. |
Mutations Made By | Dr. Gail Martin, University of California San Francisco |
When maintaining a live colony, these mice can be bred as homozygotes. Expected coat color from breeding is Albino.
When using the β-actin-cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #003376 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or wildtype for Tmem163<Tg(ACTB-cre)2Mrt> |
Frozen Mouse Embryo | FVB/N-Tmem163<Tg(ACTB-cre)2Mrt>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB/N-Tmem163<Tg(ACTB-cre)2Mrt>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB/N-Tmem163<Tg(ACTB-cre)2Mrt>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | FVB/N-Tmem163<Tg(ACTB-cre)2Mrt>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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