These H2 knock-out mice exhibit a decrease in the number of CD4 positive T cells in thymus, spleen and lymph nodes.
Christophe Benoist, Joslin Diabetes Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | H2 | histocompatibility-2, MHC |
Mice that are homozygous null for MHC class II genes H2-Ab1, H2-Aa, H2-Eb1, H2-Eb2, H2-Ea are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. MHC class II gene products (mRNA or protein) are not detected. A dramatic decrease is observed in the number of CD4 positive T cells in thymus, spleen and lymph nodes. This strain should serve as a suitable recipient of xenogenic Class II MHC transgenes allowing the engineering of mouse models of human MHC Class II-associated diseases.
This strain was developed in the laboratory of Dr. Christophe Benoist at IGBMC, Institut de Genetique et de Biologie Moleculaire et Cellulaire, Strasbourg. A 78.8 kb deletion disrupting Class II MHC genes was induced in 129S2/SvPas-derived H1 embryonic stem (ES) cells via Cre recombination. A hygro-resistance cassette was inserted at the deletion site. The deletion spans from the second exon of the H2-Ab1 gene the third exon of the H2-Ea gene. The H2-Aa, H2-Eb1 and H2-Eb2 genes are completely deleted. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were bred to C57BL/6 mice.
Allele Name | targeted deletion, H2 complex |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | (IA IE)null; ABetao; IIdelta; MHC class IIdelta; MHC II KO; MHC II-; MHC II0; MHC IIdelta; MHCII-; MHCIIdelta; MHC-IIdelta |
Gene Symbol and Name | H2, histocompatibility-2, MHC |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 17 |
Molecular Note | A 78.8 kb deletion disrupting Class II MHC genes was induced in 129S2/SvPas-derived H1 embryonic stem (ES) cells via Cre recombination. A hygro-resistance cassette was inserted at the deletion site. The deletion spans from the second exon of the H2-Ab1 gene the third exon of the H2-Ea gene. The H2-Aa, H2-Eb1 and H2-Eb2 genes are completely deleted. |
Mutations Made By | Christophe Benoist, Joslin Diabetes Center |
This strain originated and is maintained on a B6,129S background. The investigator maintains the strain by mating homozygotes. Reproduction is fair. The strain is immunodeficient and sensitive to poor microbiological conditions. Pneumocystis can be a problem. Expected coat color:Black, White Bellied Agouti.
When using the MHC II- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003374 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for H2<dlAb1-Ea> |
Frozen Mouse Embryo | B6;129S-H2<dlAb1-Ea>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129S-H2<dlAb1-Ea>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129S-H2<dlAb1-Ea>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6;129S-H2<dlAb1-Ea>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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