Overview

Also Known As:MHC II^-

This strain is currently unavailable due to replenishing of cryopreserved stocks.

These H2 knock-out mice exhibit a decrease in the number of CD4 positive T cells in thymus, spleen and lymph nodes.

Donating Investigator

Christophe Benoist, Joslin Diabetes Center

Genetic overview

Genetic Background	Generation

H2^dlAb1-Ea

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	H2	histocompatibility-2, MHC

View Genetics

Research Applications

Immunology, Inflammation and Autoimmunity Research
Internal/Organ Research
Research Tools

View All Research Applications

Details

Detailed Description

Mice that are homozygous null for MHC class II genes H2-Ab1, H2-Aa, H2-Eb1, H2-Eb2, H2-Ea are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. MHC class II gene products (mRNA or protein) are not detected. A dramatic decrease is observed in the number of CD4 positive T cells in thymus, spleen and lymph nodes. This strain should serve as a suitable recipient of xenogenic Class II MHC transgenes allowing the engineering of mouse models of human MHC Class II-associated diseases.

Development

This strain was developed in the laboratory of Dr. Christophe Benoist at IGBMC, Institut de Genetique et de Biologie Moleculaire et Cellulaire, Strasbourg. A 78.8 kb deletion disrupting Class II MHC genes was induced in 129S2/SvPas-derived H1 embryonic stem (ES) cells via Cre recombination. A hygro-resistance cassette was inserted at the deletion site. The deletion spans from the second exon of the H2-Ab1 gene the third exon of the H2-Ea gene. The H2-Aa, H2-Eb1 and H2-Eb2 genes are completely deleted. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were bred to C57BL/6 mice.

Control Suggestions

101045 B6129SF2/J

Additional Information

Considerations for Choosing Controls

Selected References

Madsen L; Labrecque N; Engberg J; Dierich A; Svejgaard A; Benoist C; Mathis D; Fugger L. 1999. Mice lacking all conventional MHC class II genes. Proc Natl Acad Sci U S A 96(18):10338-43PubMed: 10468609 MGI: J:57484

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Genetics

H2^dlAb1-Ea

Allele Symbol: H2^dlAb1-Ea

Allele Name	targeted deletion, H2 complex
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	(IA IE)^null; ABeta^o; II^delta; MHC class II^delta; MHC II KO; MHC II^-; MHC II⁰; MHC II^delta; MHCII^-; MHCII^delta; MHC-II^delta
Gene Symbol and Name	H2, histocompatibility-2, MHC
Gene Synonym(s)
Strain of Origin	129S2/SvPas
Chromosome	17
Molecular Note	A 78.8 kb deletion disrupting Class II MHC genes was induced in 129S2/SvPas-derived H1 embryonic stem (ES) cells via Cre recombination. A hygro-resistance cassette was inserted at the deletion site. The deletion spans from the second exon of the H2-Ab1 gene the third exon of the H2-Ea gene. The H2-Aa, H2-Eb1 and H2-Eb2 genes are completely deleted.
Mutations Made By	Christophe Benoist, Joslin Diabetes Center

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
- Lymphoid Tissue Defects
- Immunodeficiency
  - MHC class II deficient
Internal/Organ Research
- Lymphoid Tissue Defects
Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - MHC class II defects

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: H2^dlAb1-Ea/H2^dlAb1-Ea
B6.129S2-H2/J

immune system phenotype

increased susceptibility to bacterial infection
- following infection with either a low or high dose of IOE, mice succumb to the infection at 11 to 15 days and 7 to 9 days, respectively, compared to wild-type mice, which succumb at 14 to 17 days and 8 to 12 days, respectively
- mice are more susceptible to infection with a monocytotropic Ehrlichia bacteria from Ixodes ovatrus ticks (IOE) than wild-type mice
- mice have higher burdens of Ehrlichia bacteria in all organs following infection than do wild-type mice

mortality/aging

increased sensitivity to induced morbidity/mortality
- following infection with either a low or high dose of IOE, mice succumb to the infection at 11 to 15 days and 7 to 9 days, respectively, compared to wild-type mice, which succumb at 14 to 17 days and 8 to 12 days, respectively

The following phenotype relates to a compound genotype created using this strain

Genotype: H2^dlAb1-Ea/H2^dlAb1-Ea
involves: 129S2/SvPas * C57BL/6

hematopoietic system phenotype

abnormal immunoglobulin level

abnormal T cell number

decreased CD4-positive, alpha beta T cell number
- almost completely absent in spleen and lymph nodes
- significantly decreased number of CD4+ thymocytes

decreased IgG1 level

decreased IgG2a level

increased CD8-positive, alpha-beta T cell number

increased IgM level

mammalian phenotype

immune system phenotype
- Normal - stimulated dendritic cells produce normal amounts of IL-12 and TNF-alpha

immune system phenotype

abnormal immunoglobulin level

abnormal T cell number

decreased CD4-positive, alpha beta T cell number
- almost completely absent in spleen and lymph nodes
- significantly decreased number of CD4+ thymocytes

decreased IgG1 level

decreased IgG2a level

increased CD8-positive, alpha-beta T cell number

increased IgM level

References

Madsen L; Labrecque N; Engberg J; Dierich A; Svejgaard A; Benoist C; Mathis D; Fugger L. 1999. Mice lacking all conventional MHC class II genes. Proc Natl Acad Sci U S A 96(18):10338-43PubMed: 10468609 MGI: J:57484

Additional - H2^dlAb1-Ea related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:H2 Alternate 2
- Genotyping resources and troubleshooting
Breeding Considerations

This strain originated and is maintained on a B6,129S background. The investigator maintains the strain by mating homozygotes. Reproduction is fair. The strain is immunodeficient and sensitive to poor microbiological conditions. Pneumocystis can be a problem. Expected coat color:Black, White Bellied Agouti.
- Additional Breeding and Husbandry Support
Appearance
- white-bellied agouti
  Related Genotype: A^w/?
  
  black
  Related Genotype: a/a
Citation
When using the MHC II^- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003374 in your Materials and Methods section.

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Also Known As:MHC II-

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

H2dlAb1-Ea

Allele Symbol: H2dlAb1-Ea

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: H2dlAb1-Ea/H2dlAb1-EaB6.129S2-H2/J

immune system phenotype

mortality/aging

Genotype: H2dlAb1-Ea/H2dlAb1-Eainvolves: 129S2/SvPas * C57BL/6

hematopoietic system phenotype

mammalian phenotype

immune system phenotype

References

Additional - H2dlAb1-Ea related

Genotyping Protocols

Breeding Considerations

Appearance

Citation

Strain Interest Registration

Contact Information

Interest

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Also Known As:MHC II^-

H2^dlAb1-Ea

Allele Symbol: H2^dlAb1-Ea

Genotype: H2^dlAb1-Ea/H2^dlAb1-Ea
B6.129S2-H2/J

Genotype: H2^dlAb1-Ea/H2^dlAb1-Ea
involves: 129S2/SvPas * C57BL/6

Additional - H2^dlAb1-Ea related