These huZP3 mice contain the human ZP3 gene in place of the endogeneous mouse Zp3 gene. A zona pellucida is formed on oocytes and the females are fertile whereas mice lacking endogenous Zp3 do not form a zona pellucida and are infertile.
Jurrien Dean, NIH / NIDDK
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
The strain originated on an FVB/N background and is currently on the same background (2006).
Expressed Gene | ZP3, zona pellucida glycoprotein 3, human |
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Site of Expression |
Allele Name | transgene insertion 7812, Jurrien Dean |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | huZP3; TgN(HuZP3)3NIH |
Gene Symbol and Name | Tg(ZP3)7812Dean, transgene insertion 7812, Jurrien Dean |
Gene Synonym(s) | |
Promoter | ZP3, zona pellucida glycoprotein 3, human |
Expressed Gene | ZP3, zona pellucida glycoprotein 3, human |
Strain of Origin | FVB/N |
Chromosome | UN |
General Note | Three transgenic lines were established. This line and the line originally called TgN(HuZP3)2NIH each was estimated by Southern blot densitometry to carry 2 copies, and the line originally called TgN(HuZP3)1NIH to carry 4-6 copies of the transgene. Authors state that null-allele rescue results using the former two strains were identical. |
Molecular Note | This transgene comprises 6 kb of 5' flanking DNA from the mouse zona pellucida 3 gene ligated to the 5' half of the human zona pellucida 3 gene, including exons and introns 1-4, followed by cDNA encoded by exons 5-8 of the human gene and the bovine growth hormone polyadenylation signal. This transgenic line was estimated by Southern blot densitometry to carry two copies of the transgene. Transgene expression was shown by RNase protection analysis of multiple tissues to be ovary-specific. In situ hybridization localized expression to the cytoplasm of growing oocytes, at a level similar to that of the co-expressed endogenous mouse gene. Species-specific antibodies detect lower levels of human and mouse ZP3 in zonae pellucidae of mature transgenic oocytes than in human or wild-type mouse zonae, respectively; the total (human + mouse) approximating that in normal mouse zonae. The molecular mass of the transgenic protein suggests it undergoes post-translational modification similarly to the native human protein. |
Mutations Made By | Jurrien Dean, NIH / NIDDK |
Mice homozygous for this transgene are reported to be viable and fertile with normal reproduction. When maintaining a live colony, transgene carrier mice may be bred together, to wildtype (noncarrier) mice from the colony or to FVB/NJ inbred mice (Stock No. 001800). Alternatively, homozygous mice may be bred together.
When using the HuZP3 mouse strain in a publication, please cite the originating article(s) and include JAX stock #003335 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Homozygous for Tg(ZP3)7812Dean, 1 pair minimum |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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