FVB/N-Tg(ZP3)7812Dean/J

Stock number: 003335
Product name: HuZP3
Research areas: Reproductive Biology Research

Description

These huZP3 mice contain the human ZP3 gene in place of the endogeneous mouse Zp3 gene. A zona pellucida is formed on oocytes and the females are fertile whereas mice lacking endogenous Zp3 do not form a zona pellucida and are infertile.

Development

The strain originated on an FVB/N background and is currently on the same background (2006).

Allele

Symbol: Tg(ZP3)7812Dean
Name: transgene insertion 7812, Jurrien Dean
MGI accession ID: MGI:3056745
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(ZP3)7812Dean
Marker name: transgene insertion 7812, Jurrien Dean
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: ZP3,zona pellucida glycoprotein 3,human
Molecular note: This transgene comprises 6 kb of 5' flanking DNA from the mouse zona pellucida 3 gene ligated to the 5' half of the human zona pellucida 3 gene, including exons and introns 1-4, followed by cDNA encoded by exons 5-8 of the human gene and the bovine growth hormone polyadenylation signal. This transgenic line was estimated by Southern blot densitometry to carry two copies of the transgene. Transgene expression was shown by RNase protection analysis of multiple tissues to be ovary-specific. In situ hybridization localized expression to the cytoplasm of growing oocytes, at a level similar to that of the co-expressed endogenous mouse gene. Species-specific antibodies detect lower levels of human and mouse ZP3 in zonae pellucidae of mature transgenic oocytes than in human or wild-type mouse zonae, respectively; the total (human + mouse) approximating that in normal mouse zonae. The molecular mass of the transgenic protein suggests it undergoes post-translational modification similarly to the native human protein.
General note: Three transgenic lines were established. This line and the line originally called TgN(HuZP3)2NIH each was estimated by Southern blot densitometry to carry 2 copies, and the line originally called TgN(HuZP3)1NIH to carry 4-6 copies of the transgene. Authors state that null-allele rescue results using the former two strains were identical.