These NotchIC-9 mice express a transgene containing an activated form of Notch under the control of the thymocyte specific Lck proximal promoter and exhibit an increase in mature CD8 lineage thymocytes.
Dr. Ellen Robey, Univ of California, Berkeley
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Inserted expressed sequence) |
C57BL/6-TgN(LckNotch1)9Erob mice are viable and fertile. They express an activated form of Notch under the control of the thymocyte specific Lck proximal promoter. Transgenic mice have a 10-fold increase in both absolute number and percentage of mature CD8 lineage thymocytes compared to non-transgenic littermates, with a corresponding decrease in CD4 lineage cells. Activated Notch does not affect the proliferative capacity or lifespan of immature CD4+CD8+ thymocytes, nor does it lead to inappropriate proliferation of mature thymocytes. Instead, activated Notch appears to create a bias towards CD8 T cell development while inhibiting CD4 T cell development. These mice are useful for studying the role of Notch in the CD4/CD8 lineage decision in thymocytes.
Expressed Gene | Notch1, notch 1, mouse, laboratory |
---|---|
Site of Expression |
Allele Name | transgene insertion 9, Ellen Robey |
---|---|
Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | lck-Nic |
Gene Symbol and Name | Tg(LckNotch1)9Erob, transgene insertion 9, Ellen Robey |
Gene Synonym(s) | |
Promoter | Lck, lymphocyte protein tyrosine kinase, mouse, laboratory |
Expressed Gene | Notch1, notch 1, mouse, laboratory |
Strain of Origin | (C57BL/6 x CBA/J)F2 |
Chromosome | UN |
Molecular Note | The transgene expresses an activated form of Notch fused to a FLAG epitope under the control of the thymocyte specific Lck proximal promoter. |
Mutations Made By | Dr. Ellen Robey, Univ of California, Berkeley |
Homozygotes are viable and fertile. The donating laboratory used C57BL/6 female x heterozygote male crosses. Young breeders are recommended as older mice develop tumors.
When using the NotchIC-9 mouse strain in a publication, please cite the originating article(s) and include JAX stock #003332 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Carrier or non-carrier for Tg(LckNotch1)9Erob |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.