C57BL/6-Tg(LckNotch1)9Erob/J

Stock number: 003332
Product name: NotchIC-9

Description

These NotchIC-9 mice express a transgene containing an activated form of Notch under the control of the thymocyte specific Lck proximal promoter and exhibit an increase in mature CD8 lineage thymocytes.

Detailed description

C57BL/6-TgN(LckNotch1)9Erob mice are viable and fertile. They express an activated form of Notch under the control of the thymocyte specific Lck proximal promoter. Transgenic mice have a 10-fold increase in both absolute number and percentage of mature CD8 lineage thymocytes compared to non-transgenic littermates, with a corresponding decrease in CD4 lineage cells. Activated Notch does not affect the proliferative capacity or lifespan of immature CD4⁺CD8⁺ thymocytes, nor does it lead to inappropriate proliferation of mature thymocytes. Instead, activated Notch appears to create a bias towards CD8 T cell development while inhibiting CD4 T cell development. These mice are useful for studying the role of Notch in the CD4/CD8 lineage decision in thymocytes.

Allele

Symbol: Tg(LckNotch1)9Erob
Name: transgene insertion 9, Ellen Robey
MGI accession ID: MGI:3056811
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(LckNotch1)9Erob
Marker name: transgene insertion 9, Ellen Robey
Chromosome: UN
Strain of origin: (C57BL/6 x CBA/J)F2
Expressed genes: Notch1,notch 1,mouse, laboratory
Molecular note: The transgene expresses an activated form of Notch fused to a FLAG epitope under the control of the thymocyte specific Lck proximal promoter.