C57BL/6-Tg(Cd8aCd8b)3Erob/J

Stock number: 003331
Product name: CD8

Description

These CD8 mice contain a transgene constitutively expressing CD8.1 under the control of the T cell-specific CD2 promoter.

Detailed description

Homozygous mice constitutively express CD8.1 under the control of the T cell-specific CD2 promoter. Homozygotes are viable and fertile. CD8.1 differs from the CD8.2 allele expressed in the C57BL/6 mouse strain, allowing differentiation between the endogenous protein and the transgene product using monoclonal antibodies. This transgenic line was created to study the mechanisms of T cell selection and lineage commitment in the thymus.

Allele

Symbol: Tg(Cd8aCd8b)3Erob
Name: transgene insertion 3, Ellen Robey
MGI accession ID: MGI:3056809
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Cd8aCd8b)3Erob
Marker name: transgene insertion 3, Ellen Robey
Chromosome: UN
Strain of origin: (C57BL/6 x DBA/2)F2
Expressed genes: Cd8a,CD8a molecule,rat
Molecular note: The murine Cd8a and Cd8b1 coding sequences were placed under the control of the T cell-specific CD2 promoter. Six copies of the transgene incorporated into the genome. The resulting heterodimer complex expressed was the "CD8.1" variant that differs from the "CD8.2" variant complex normally expressed in C57BL/6 mice. Transgenic expression was detected on almost all thymocytes and T cells as determined by flow cytometry using antibodies that can distinguish the two CD8 variants.