These CD8 mice contain a transgene constitutively expressing CD8.1 under the control of the T cell-specific CD2 promoter.
Dr. Ellen Robey, Univ of California, Berkeley
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Homozygous mice constitutively express CD8.1 under the control of the T cell-specific CD2 promoter. Homozygotes are viable and fertile. CD8.1 differs from the CD8.2 allele expressed in the C57BL/6 mouse strain, allowing differentiation between the endogenous protein and the transgene product using monoclonal antibodies. This transgenic line was created to study the mechanisms of T cell selection and lineage commitment in the thymus.
Expressed Gene | Cd8a, CD8a molecule, rat |
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Site of Expression |
Allele Name | transgene insertion 3, Ellen Robey |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(Cd8aCd8b)3Erob, transgene insertion 3, Ellen Robey |
Gene Synonym(s) | |
Promoter | CD2, CD2 molecule, human |
Expressed Gene | Cd8a, CD8a molecule, rat |
Strain of Origin | (C57BL/6 x DBA/2)F2 |
Chromosome | UN |
Molecular Note | The murine Cd8a and Cd8b1 coding sequences were placed under the control of the T cell-specific CD2 promoter. Six copies of the transgene incorporated into the genome. The resulting heterodimer complex expressed was the "CD8.1" variant that differs from the "CD8.2" variant complex normally expressed in C57BL/6 mice. Transgenic expression was detected on almost all thymocytes and T cells as determined by flow cytometry using antibodies that can distinguish the two CD8 variants. |
Mutations Made By | Dr. Ellen Robey, Univ of California, Berkeley |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the CD8 mouse strain in a publication, please cite the originating article(s) and include JAX stock #003331 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Homozygous for Tg(Cd8aCd8b)3Erob |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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