STOCK Tg(Cebpb-tTA)5Bjd/J

Stock number: 003272

Description

This strain has been discontinued. Please see Stock No. 003563

Detailed description

The tet regulatory system in which doxycycline (dox) acts as an inducer of specifically engineered RNA polymerase II promoters was transferred into transgenic mice. Administration of dox rapidly induces the synthesis of the indicator enzyme luciferase whose activity rises several orders of magnitude over a period of hours. A comparable regulatory potential exists in the tet regulatory system where dox prevents transcription activation. Both the rtTA and the tTA systems provide true genetic switches capable of quantitatively controlling individual gene activities in animals in a highly tissue-specific manner providing proper combinations of promoter and chromosomal integration sites are met. This is a mouse line where tetracycline controlled transactivators are synthesized tissue-specifically in the liver. Directing the synthesis of the tetracycline-controlled transactivator (tTA) to the liver leads to highly specific regulation in hepatocytes where, in the presence of dox, less than one molecule of luciferase is detected per cell, in contrast to more than 10⁵-fold of luciferase in the absence of the antibiotic. Dox concentration may be controlled via administration in the water supply of the animals.

Development

Stock No. 003272 (STOCK Tg(tTALap)5Bjd/J) was made by Dr. Hermann Bujard on a mixed FVB/N and NMRI-outbred background. This strain was distributed from The Jackson Laboratory Repository from January 2000-February 2001 and is no longer available through this resource. While available as a live colony, The Jackson Laboratory bred these LAP-tTA mice to C57BL/6J to generate a congenic strain (see Stock No. 003563) that is available through this resource.

Allele

Symbol: Tg(Cebpb-tTA)5Bjd
Name: transgene insertion 5, Hermann Bujard
MGI accession ID: MGI:3056818
Generation method: Transgenic
Attributes: Transactivator
Marker symbol: Tg(Cebpb-tTA)5Bjd
Marker name: transgene insertion 5, Hermann Bujard
Chromosome: UN
Strain of origin: NMRI
Expressed genes: tTA,tetracycline-controlled transactivator,E. coli
Site of expression: Expresses tTA at high levels in the liver; see Stock No. 003563
Molecular note: This transgene contains the gene encoding the tetracycline regulated transactivator protein (tTA) driven by the rat Cebpb promoter (previously called liver-enriched activator protein).
General note: Transgenic mice on a C57BL/6J background are viable and fertile, and express tTA in the liver.