B6;129S2-Cdkn1a^tm1Tyj/J

Stock number: 003263
Product name: P21^-
Research areas: Cancer Research, Cardiovascular Research, Cell Biology Research

Description

Mouse embryo fibroblasts from homozygous Cdkn1a^tm1Tyj mutants have impaired ability to undergo G1 arrest following DNA damage.

Detailed description

Homozygotes are viable and fertile. p21, the product of the Cdkn1a gene, belongs to a family of regulators, known as cyclin-dependent kinase inhibitors, which modulate progression through the cell cycle. Following serum restimulation, expression of p21 is superinducible by cycloheximide in wild-type but not in p53-deficient cells. p53 appears to play a critical role in p21 induction following DNA damage. p21 can be regulated independently of p53 during normal tissue development, following serum stimulation, and during cellular differentiation.

Allele

Symbol: Cdkn1a^tm1Tyj
Name: targeted mutation 1, Tyler Jacks
MGI accession ID: MGI:1933751
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Cdkn1a
Marker name: cyclin-dependent kinase inhibitor 1A (P21)
Chromosome: 17
Strain of origin: 129S2/SvPas
Molecular note: The coding sequences were replaced by a neomycin cassette. No protein was detected by immunoprecipitation of ES cell lysates in which both alleles were disrupted.