Mouse embryo fibroblasts from homozygous Cdkn1atm1Tyj mutants have impaired ability to undergo G1 arrest following DNA damage.
Dr. Tyler Jacks, Massachusetts Institute of Technology
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Cdkn1a | cyclin-dependent kinase inhibitor 1A (P21) |
Homozygotes are viable and fertile. p21, the product of the Cdkn1a gene, belongs to a family of regulators, known as cyclin-dependent kinase inhibitors, which modulate progression through the cell cycle. Following serum restimulation, expression of p21 is superinducible by cycloheximide in wild-type but not in p53-deficient cells. p53 appears to play a critical role in p21 induction following DNA damage. p21 can be regulated independently of p53 during normal tissue development, following serum stimulation, and during cellular differentiation.
Allele Name | targeted mutation 1, Tyler Jacks |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Cdkn1a-; p21-; p21cip- |
Gene Symbol and Name | Cdkn1a, cyclin-dependent kinase inhibitor 1A (P21) |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 17 |
Molecular Note | The coding sequences were replaced by a neomycin cassette. No protein was detected by immunoprecipitation of ES cell lysates in which both alleles were disrupted. |
Mutations Made By | Dr. Tyler Jacks, Massachusetts Institute of Technology |
When using the P21- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003263 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Cdkn1a<tm1Tyj>/ |
Frozen Mouse Embryo | B6;129S2-Cdkn1a<tm1Tyj>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S2-Cdkn1a<tm1Tyj>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S2-Cdkn1a<tm1Tyj>/J | $3373.50 |
Frozen Mouse Embryo | B6;129S2-Cdkn1a<tm1Tyj>/J | $3373.50 |
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