These Fgf2 knock-out mice exhibit decreased vascular smooth muscle contractility, low blood pressure and thrombocytosis.
Thomas Doetschman, University of Arizona
Mice homozygous for the Fgf2tm1Doe targeted-mutant allele have low blood pressure, presumably resulting from low vascular tone, increased megakaryocyte colony stimulation activity-induced megakaryocyte colony formation; increased platelet counts, and decreased IL3-induced colony formation. Vessel layer hypertrophy following vessel injury is not altered in the absence of FGF2. Cardiac hypertrophic response to induced high blood pressure is severely blunted in the absence of FGF2. Both sexes of the mutant exhibit no discernible morphologic or behavioral defects and have a normal life span. Both sexes are fertile and fecundity is normal.
In the mutant allele, an Hprt minigene replaces 0.5kb of sequence containing 121bp of the proximal promoter region and exon 1. The targeting construct was electroporated into 129P2/OlaHsd-derived E14TG2a embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6-derived blastocysts, and the resulting chimeric mice were bred to outbred Black Swiss mice.
|Allele Name||targeted mutation 1, Thomas Doetschman|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||FGF-2-; Fgf2-|
|Gene Symbol and Name||Fgf2, fibroblast growth factor 2|
|Gene Synonym(s)||BFGF; FGF-2; FGFB; Fgf-2; Fgf-2; Fgfb; Fgfb; HBGF-2; bFGF; fibroblast growth factor, basic|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||An Hprt minigene replaced 0.5kb of sequence containing 121bp of the proximal promoter region and exon 1. Northern blot analysis of E13.5 embryos demonstrated that no mRNA containing exon 2 and 3 sequences is detectable in homozygous mutant mice. Western blot analysis on brain tissue from homozygous mutant mice showed that the protein is absent.|
|Mutations Made By|| |
Thomas Doetschman, University of Arizona
Homozygotes are viable and fertile. Prior to its arrival at The Jackson Laboratory, the donating investigator maintained the strain as an advanced intercross line by breeding non-sibling heterozygotes to avoid inbreeding. At The Jackson Laboratory, the strain was maintained similarly by intercrossing non-sibling heterozygotes. The donating investigator indicated that mice that carry this mutation may manifest developmental defects on a C57BL/6 background. Expected coat color from breeding: agouti and chinchilla.
When using the Fgf2 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #003256 in your Materials and Methods section.
|Heterozygous or Homozygous or Wild-type for Fgf2<tm1Doe>|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
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|Frozen Mouse Embryo||$2,595.00 per straw or vial|
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