B6;129-Hbb-b1^tm1Unc Hbb-b2^tm1Unc/J

Stock number: 003253
Product name: Β-thalassemic
Research areas: Hematological Research

Description

These mice carry a double knock-out of the Hbb-b1 and Hbb-b2 adult Β-globin genes with a phenotype similar to Cooley anemia in humans.

Detailed description

Homozygous mice die perinatally. Heterozygous mice show "characteristics typical of severe thalassemia" and are fertile.

Development

A targeting vector was designed to delete Hbb-b1 and Hbb-b2 (closely linked on Chromosome 7) using a two-step "plug and socket" method which leaves an hprt gene integrated in the genome. The construct was electroporated into 129P2/OlaHsd-derived E14TG2a embryonic stem (ES) cells. Documentation from 1996-1997 report the mice were backcrossed to C57BL/6J for at least 3 generations prior to arrival in The Jackson Laboratory Repository vivarium as Stock No. 002683. One additional backcross generation to C57BL/6J was performed and then one male was bred to B6129SF1/J females (Stock No. 101043) to establish this B6;129 colony as Stock No. 003253. At least one (perhaps several) breedings to Stock No. 101043 have subsequently occurred.

Allele

Symbol: Hbb-b2^tm1Unc
Name: targeted mutation 1, University of North Carolina
MGI accession ID: MGI:1857296
Generation method: Targeted
Attributes: Null/Knockout
Synonyms: Hbb^th3; Hbb^th-3
Marker symbol: Hbb-b2
Marker name: hemoglobin, beta adult minor chain
Marker synonyms: beta min; beta minor globin; beta2; beta-globin; CD113t-C; ECYT6
Chromosome: 7
Strain of origin: 129P2/OlaHsd
Molecular note: Hbb-b1 and Hbb-b2 were deleted using a two-step "plug and socket" method which leaves an hprt gene integrated in the genome.

Allele

Symbol: Hbb-b1^tm1Unc
Name: targeted mutation 1, University of North Carolina
MGI accession ID: MGI:1857295
Generation method: Targeted
Attributes: Null/Knockout
Synonyms: Hbb^th3; Hbb^th-3
Marker symbol: Hbb-b1
Marker name: hemoglobin, beta adult major chain
Marker synonyms: beta maj; beta major globin; beta1; beta-globin; CD113t-C; ECYT6; MommeD7
Chromosome: 7
Strain of origin: 129P2/OlaHsd
Molecular note: Hbb-b1 and Hbb-b2 were deleted using a two-step "plug and socket" method which leaves an hprt gene integrated in the genome.