IL-1 receptor KO mice exhibit altered innate immune and inflammatory responses and show multiple effects on physiological systems and behavior.
Dr. Jacques Peschon, Amgen
Interleukin 1 receptor, type I, is a receptor for interleukin 1 (IL1), which affects many cytokine induced immune and inflammatory responses. Mice homozygous for the Il1rtm1Imx targeted mutation therefore fail to respond to IL1 and exhibit an altered immune response to many different target proteins. Some effects seen from knocking out this receptor include defective responses to certain inflammatory agents such as turpentine and altered response to various pathogenic organisms such as S. aureus and C. pneumoniae.
Other observed effects include enhanced glucose homeostasis, enhanced insulin sensitivity of adipose tissue, reduction in diet-induced systemic atherosclerosis, improved restoration of normal skin architecture in wounds, increased incidence of acute convulsive seizures induced by pentylenetetrazole, and a decrease in anxiety-related behaviors.
A null mutation in Il1r1 was generated by homologous recombination in 129/SvEv AB1 ES cells using a replacement vector in which a 2.4 kb EcoR1-Pst1 fragment encompassing two exons was replaced with a PGKneo cassette. Targeted mutant mice were backcrossed 5 times to C57BL/6 prior to importation (see SNP note below).
A 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. One the 43 markers on Chromosome 8 was segregating with 129. Also, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
|Allele Name||targeted mutation 1, Immunex Research and Development Corporation|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||IL-1R1-; IL-1R-; IL-1RI KO; IL-R1-; Il1r1tm1jmx; Il1rtm1Imx; RIko|
|Gene Symbol and Name||Il1r1, interleukin 1 receptor, type I|
|Gene Synonym(s)||CD121A; CD121a; D2S1473; IL-1 receptor alpha chain; IL-1R-alpha; IL-1R1; IL-iR; IL1R; IL1RA; Il1r-1; Il1r-1; P80|
|Strain of Origin||129S7/SvEvBrd-Hprt<+>|
|Molecular Note||A neomycin resistance cassette replaced two exons of the gene, which encode amino acids 4 - 225 of the mature protein.|
|Mutations Made By|| |
Dr. Jacques Peschon, Amgen
This strain may be maintained by homozygous sibling matings. Expected coat color from breeding is black.
When using the Il1r1- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003245 in your Materials and Methods section.
|Homozygous for Il1r1<tm1Imx>|
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