B10.PL-H2^u H2-T18^a/(73NS)Sn-Tg(TCRB)C14Jg/J

Stock number: 003200
Product name: TCR β chain
Research areas: Research Tools

Description

These TCR Β chain mice express a transgene containing a functionally rearranged Tcrb gene.

Detailed description

TCR-transgenic mice exhibit the generation of an unusual population of CD4^-CD8^-TCR⁺ thymocytes and the absence of gama delta cells. Transgenic TCR beta chain causes immature T cells to accelerate differentiation into the alpha-beta lineage and thus inhibits the generation of gamma-delta cells. In combination with the transgenic strain TCR alpha, it can be used as a model for Multiple Sclerosis.

Allele

Symbol: Tg(TCRB)C14Jg
Name: transgene insertion C14, Joan M Goverman
MGI accession ID: MGI:3055339
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(TCRB)C14Jg
Marker name: transgene insertion C14, Joan M Goverman
Chromosome: UN
Strain of origin: C57BL/6 x DBA/2
Expressed genes: Tcrb,T cell receptor beta chain,mouse, laboratory
Molecular note: A functionally rearranged Tcrb gene was used in the construction of this transgene A recombined Tcrb gene with Vbeta8.2/Dbeta2/Jbeta2.6 gene segments was cloned from the B10.PL hybridoma "172.10". This hybridoma was specific for the 11 amino-terminal residues of myelin basic protein (MBP). Crossing this line with the Tg(TCRA)B1Jg line produces a T cell with a transgenic TCR that has affinity for the first 11 amino acids of MBP. In these doubly transgenic mice, almost all of the CD3⁺ cells express the transgenic Tcrb gene.

Allele

Symbol: H2^u
Name: u variant
MGI accession ID: MGI:3834991
Generation method: Not Applicable
Marker symbol: H2
Marker name: histocompatibility-2, MHC
Chromosome: 17
Strain of origin: Not Applicable
General note: The q variant has been observed in the PL/J strain.

Allele

Symbol: H2-T18^a
Name: a variant
MGI accession ID: MGI:4820507
Generation method: Not Applicable
Marker symbol: H2-T18
Marker name: histocompatibility 2, T region locus 18
Chromosome: 17
Strain of origin: Not Applicable
Molecular note: The allele H2-T18^a determines presence of the antigenic specificities TL.1, 2, 3, 5, 6, 7 and occurs in strains A, C57BR, C58, SJL, and others.

Allele

MGI accession ID: MGI:95950
Marker symbol: H2-T18
Marker name: histocompatibility 2, T region locus 18
Chromosome: 17

Allele

MGI accession ID: MGI:95894
Marker symbol: H2
Marker name: histocompatibility-2, MHC
Chromosome: 17