Overview

Also Known As:TCR β chain

These TCR Β chain mice express a transgene containing a functionally rearranged Tcrb gene.

Donating Investigator

Dr. Joan M. Goverman, University of Washington

Genetic overview

Genetic Background	Generation

Tg(TCRB)C14Jg

Allele Type
Transgenic (Inserted expressed sequence)

H2^u

Allele Type	Gene Symbol	Gene Name
Not Applicable	H2	histocompatibility-2, MHC

H2-T18^a

Allele Type	Gene Symbol	Gene Name
Not Applicable	H2-T18	histocompatibility 2, T region locus 18

Marker(s)

	Marker Symbol	Marker Name
	H2	histocompatibility-2, MHC
	H2-T18	histocompatibility 2, T region locus 18

View Genetics

Research Applications

Research Tools

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

TCR-transgenic mice exhibit the generation of an unusual population of CD4^-CD8^-TCR⁺ thymocytes and the absence of gama delta cells. Transgenic TCR beta chain causes immature T cells to accelerate differentiation into the alpha-beta lineage and thus inhibits the generation of gamma-delta cells. In combination with the transgenic strain TCR alpha, it can be used as a model for Multiple Sclerosis.

Expression Data

Expressed Gene	Tcrb, T cell receptor beta chain, mouse, laboratory
Site of Expression
Site of Expression
Site of Expression

Control Suggestions

Noncarrier
000458 B10.PL-H2^u H2-T18^a/(73NS)SnJ

Additional Information

Considerations for Choosing Controls

Selected References

Brabb T; Rubicz R; Mannikko V; Goverman J. 1997. Separately expressed T cell receptor alpha and beta chain transgenes exert opposite effects on T cell differentiation and neoplastic transformation. Eur J Immunol 27(11):3039-48PubMed: 9394835 MGI: J:92992

View All References

Genetics

Tg(TCRB)C14Jg

Allele Symbol: Tg(TCRB)C14Jg

Allele Name	transgene insertion C14, Joan M Goverman
Allele Type	Transgenic (Inserted expressed sequence)
Allele Synonym(s)
Gene Symbol and Name	Tg(TCRB)C14Jg, transgene insertion C14, Joan M Goverman
Gene Synonym(s)
Promoter	Tcrb, T cell receptor beta chain, mouse, laboratory
Expressed Gene	Tcrb, T cell receptor beta chain, mouse, laboratory
Strain of Origin	C57BL/6 x DBA/2
Chromosome	UN
Molecular Note	A functionally rearranged Tcrb gene was used in the construction of this transgene A recombined Tcrb gene with Vbeta8.2/Dbeta2/Jbeta2.6 gene segments was cloned from the B10.PL hybridoma "172.10". This hybridoma was specific for the 11 amino-terminal residues of myelin basic protein (MBP). Crossing this line with the Tg(TCRA)B1Jg line produces a T cell with a transgenic TCR that has affinity for the first 11 amino acids of MBP. In these doubly transgenic mice, almost all of the CD3⁺ cells express the transgenic Tcrb gene.
Mutations Made By	Dr. Joan Goverman, University of Washington

H2^u

Allele Symbol: H2^u

Allele Name	u variant
Allele Type	Not Applicable
Allele Synonym(s)
Gene Symbol and Name	H2, histocompatibility-2, MHC
Gene Synonym(s)
Strain of Origin	Not Applicable
Chromosome	17
General Note	The q variant has been observed in the PL/J strain.

H2-T18^a

Allele Symbol: H2-T18^a

Allele Name	a variant
Allele Type	Not Applicable
Allele Synonym(s)	Tla^a
Gene Symbol and Name	H2-T18, histocompatibility 2, T region locus 18
Gene Synonym(s)
Strain of Origin	Not Applicable
Chromosome	17
Molecular Note	The allele H2-T18^a determines presence of the antigenic specificities TL.1, 2, 3, 5, 6, 7 and occurs in strains A, C57BR, C58, SJL, and others.

H2

Marker Symbol: H2

Marker Synonym(s)
Chromosome(s)	17

H2-T18

Marker Symbol: H2-T18

Marker Synonym(s)
Chromosome(s)	17

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - T Cell Receptor Transgenics

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: H2^u/H2^u Tg(TCRB)C14Jg/0
B10.PL-H2 H2-T18/(73NS)Sn-Tg(TCRB)C14Jg/J

hematopoietic system phenotype

abnormal double-negative T cell morphology

abnormal double-positive T cell morphology
- the appearance of double-positive T cells in the thymus first occurs on E15 compared to E17 for controls

absent gamma-delta T cells
- gamma-delta T cells are not detected in the thymus of E18 mice

immune system phenotype

abnormal double-negative T cell morphology
- a greater proportion of the double-negative thymocytes present in E16 and E17 thymi are in the most mature (c-kit^-CD44^-CD25^-) stage compared to controls

abnormal double-positive T cell morphology
- the appearance of double-positive T cells in the thymus first occurs on E15 compared to E17 for controls

absent gamma-delta T cells
- gamma-delta T cells are not detected in the thymus of E18 mice

References

Brabb T; Rubicz R; Mannikko V; Goverman J. 1997. Separately expressed T cell receptor alpha and beta chain transgenes exert opposite effects on T cell differentiation and neoplastic transformation. Eur J Immunol 27(11):3039-48PubMed: 9394835 MGI: J:92992

Additional - H2 related

Additional - H2^u related

Additional - H2-T18 related

Additional - H2-T18^a related

Additional - Tg(TCRB)C14Jg related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Tg(TCRB)C14Jg
- Genotyping resources and troubleshooting
Breeding Considerations

Homozygotes are not viable. The strain is maintained by mating heterozygotes. The expected coat color from breeding is black.
- Additional Breeding and Husbandry Support
Appearance
- black
  Related Genotype: a/a
Citation
When using the TCR β chain mouse strain in a publication, please cite the originating article(s) and include JAX stock #003200 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Hemizygous or Non carrier for (73NS)Sn-Tg(TCRB)C14Jg

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

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Also Known As:TCR β chain

Donating Investigator

Genetic overview

Marker(s)

Research Applications

Base Price

Detailed Description

Expression Data

Control Suggestions

Additional Information

Selected References

Tg(TCRB)C14Jg

Allele Symbol: Tg(TCRB)C14Jg

H2u

Allele Symbol: H2u

H2-T18a

Allele Symbol: H2-T18a

H2

Marker Symbol: H2

H2-T18

Marker Symbol: H2-T18

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: H2u/H2u Tg(TCRB)C14Jg/0B10.PL-H2 H2-T18/(73NS)Sn-Tg(TCRB)C14Jg/J

immune system phenotype

References

Additional - H2 related

Additional - H2u related

Additional - H2-T18 related

Additional - H2-T18a related

Additional - Tg(TCRB)C14Jg related

Genotyping Protocols

Breeding Considerations

Appearance

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

H2^u

Allele Symbol: H2^u

H2-T18^a

Allele Symbol: H2-T18^a

Genotype: H2^u/H2^u Tg(TCRB)C14Jg/0
B10.PL-H2 H2-T18/(73NS)Sn-Tg(TCRB)C14Jg/J

Additional - H2^u related

Additional - H2-T18^a related