These TCR Β chain mice express a transgene containing a functionally rearranged Tcrb gene.
Dr. Joan M. Goverman, University of Washington
Genetic Background | Generation |
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|
Allele Type |
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Transgenic (Inserted expressed sequence) |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Not Applicable | H2 | histocompatibility-2, MHC |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Not Applicable | H2-T18 | histocompatibility 2, T region locus 18 |
Marker Symbol | Marker Name | |
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H2 | histocompatibility-2, MHC | |
H2-T18 | histocompatibility 2, T region locus 18 |
TCR-transgenic mice exhibit the generation of an unusual population of CD4-CD8-TCR+ thymocytes and the absence of gama delta cells. Transgenic TCR beta chain causes immature T cells to accelerate differentiation into the alpha-beta lineage and thus inhibits the generation of gamma-delta cells. In combination with the transgenic strain TCR alpha, it can be used as a model for Multiple Sclerosis.
Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
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Site of Expression | |
Site of Expression | |
Site of Expression |
Allele Name | transgene insertion C14, Joan M Goverman |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(TCRB)C14Jg, transgene insertion C14, Joan M Goverman |
Gene Synonym(s) | |
Promoter | Tcrb, T cell receptor beta chain, mouse, laboratory |
Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory |
Strain of Origin | C57BL/6 x DBA/2 |
Chromosome | UN |
Molecular Note | A functionally rearranged Tcrb gene was used in the construction of this transgene A recombined Tcrb gene with Vbeta8.2/Dbeta2/Jbeta2.6 gene segments was cloned from the B10.PL hybridoma "172.10". This hybridoma was specific for the 11 amino-terminal residues of myelin basic protein (MBP). Crossing this line with the Tg(TCRA)B1Jg line produces a T cell with a transgenic TCR that has affinity for the first 11 amino acids of MBP. In these doubly transgenic mice, almost all of the CD3+ cells express the transgenic Tcrb gene. |
Mutations Made By | Dr. Joan Goverman, University of Washington |
Allele Name | a variant |
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Allele Type | Not Applicable |
Allele Synonym(s) | Tlaa |
Gene Symbol and Name | H2-T18, histocompatibility 2, T region locus 18 |
Gene Synonym(s) | |
Strain of Origin | Not Applicable |
Chromosome | 17 |
Molecular Note | The allele H2-T18a determines presence of the antigenic specificities TL.1, 2, 3, 5, 6, 7 and occurs in strains A, C57BR, C58, SJL, and others. |
Marker Synonym(s) | |
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Chromosome(s) | 17 |
Marker Synonym(s) | |
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Chromosome(s) | 17 |
Homozygotes are not viable. The strain is maintained by mating heterozygotes. The expected coat color from breeding is black.
When using the TCR β chain mouse strain in a publication, please cite the originating article(s) and include JAX stock #003200 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for (73NS)Sn-Tg(TCRB)C14Jg |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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