C57BL/6-Tg(PRG3)9Wlad/J

Stock number: 003186
Product name: TGM-PRG-3
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

These TGM-PRG-3 mice express a transgene containing the human poliovirus receptor.

Detailed description

Mice carrying either the PRG3 (this strain) or PRG1 (Stock No. 003185) transgene provides a model for testing oral vaccines before distribution The transgene copy number is 10 in the TGM-PRG-3 mouse line and one in the TGM-PRG-1 mouse line. The two lines are similar in their susceptibility to poliovirus. The transgenic mice can be infected with either Type 1 or Type 2 human polio virus, but are not as susceptible to infection with Type 3 virus. They may also be used as a model to study mucosal immunity. According to the donating investigator homozygotes are less susceptible to polio virus.

Allele

Symbol: Tg(PRG3)9Wlad
Name: transgene insertion 9, Wyeth Lederle/Anne Deatly
MGI accession ID: MGI:3055289
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(PRG3)9Wlad
Marker name: transgene insertion 9, Wyeth Lederle/Anne Deatly
Chromosome: UN
Strain of origin: (C57BL/6J x CBA/J)F1
Expressed genes: PVR,poliovirus receptor,human
Molecular note: This line carries ten copies of the human poliovirus receptor transgene (PVR). The human polio virus receptor is a member of the Igg superfamily. Northern and Western blot analyses detected expression of the transgene at levels three times higher in mice carrying this transgene, compared to levels in transgenic mice carrying a single copy of human PVR.