These TGM-PRG-3 mice express a transgene containing the human poliovirus receptor.
Dr. Anne M. Deatly, Wyeth Lederle Vaccines & Pediatrics
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
Mice carrying either the PRG3 (this strain) or PRG1 (Stock No. 003185) transgene provides a model for testing oral vaccines before distribution The transgene copy number is 10 in the TGM-PRG-3 mouse line and one in the TGM-PRG-1 mouse line. The two lines are similar in their susceptibility to poliovirus. The transgenic mice can be infected with either Type 1 or Type 2 human polio virus, but are not as susceptible to infection with Type 3 virus. They may also be used as a model to study mucosal immunity. According to the donating investigator homozygotes are less susceptible to polio virus.
Expressed Gene | PVR, poliovirus receptor, human |
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Site of Expression |
Allele Name | transgene insertion 9, Wyeth Lederle/Anne Deatly |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | PRG3; TGM-PRG-3 |
Gene Symbol and Name | Tg(PRG3)9Wlad, transgene insertion 9, Wyeth Lederle/Anne Deatly |
Gene Synonym(s) | |
Promoter | PVR, poliovirus receptor, human |
Expressed Gene | PVR, poliovirus receptor, human |
Strain of Origin | (C57BL/6J x CBA/J)F1 |
Chromosome | UN |
Molecular Note | This line carries ten copies of the human poliovirus receptor transgene (PVR). The human polio virus receptor is a member of the Igg superfamily. Northern and Western blot analyses detected expression of the transgene at levels three times higher in mice carrying this transgene, compared to levels in transgenic mice carrying a single copy of human PVR. |
Mutations Made By | Dr. Anne Deatly, Wyeth Lederle Vaccines & Pediatrics |
The strain may be maintained by mating hemizygous males or females with C57BL/6J partners. It may also be maintained by mating homozygous males or females with C57BL/6J partners.
When using the TGM-PRG-3 mouse strain in a publication, please cite the originating article(s) and include JAX stock #003186 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(PRG3)9Wlad |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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