These Il5 knock-out mice exhibit lack of eosinophilia response to infection and impaired CD5+ B-1 cell development.
Dr. Manfred Kopf, ETH Zurich
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Il5 | interleukin 5 |
Mice homozygous for the Il5tm1Kopf targeted mutation are viable and fertile. Surprisingly, IL5 deficient mice show no defects in conventional B (B-2) cells, T cell dependent antibody production, or cytotoxic T cell responses. Eosinophilia response to infection is lacking in homozygous mutant mice. They also exhibit impaired CD5+ B-1 cell development.
A targeting vector containing the neomycin resistance gene was used to disrupt exon 3. The construct was electroporated into C57BL/6J-derived BL/6-II embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6J.
Allele Name | targeted mutation 1, Manfred A Kopf |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | IL-5-; IL-5null |
Gene Symbol and Name | Il5, interleukin 5 |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J |
Chromosome | 11 |
Molecular Note | A neomycin resistance cassette was inserted into exon 3 of the gene. |
Mutations Made By | Dr. Manfred Kopf, ETH Zurich |
The strain is maintained by breeding homozygous mutants. The expected coat color from breeding is black.
When using the IL-5- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003175 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Il5<tm1Kopf> |
Frozen Mouse Embryo | C57BL/6-Il5<tm1Kopf>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Il5<tm1Kopf>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Il5<tm1Kopf>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Il5<tm1Kopf>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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