Mice homozygous for the Il5tm1Kopf targeted mutation are viable and fertile. Surprisingly, IL5 deficient mice show no defects in conventional B (B-2) cells, T cell dependent antibody production, or cytotoxic T cell responses. Eosinophilia response to infection is lacking in homozygous mutant mice. They also exhibit impaired CD5+ B-1 cell development.
A targeting vector containing the neomycin resistance gene was used to disrupt exon 3. The construct was electroporated into C57BL/6J-derived BL/6-II embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6J.
|Allele Name||targeted mutation 1, Manfred A Kopf|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||IL-5-; IL-5null|
|Gene Symbol and Name||Il5, interleukin 5|
|Strain of Origin||C57BL/6J|
|Molecular Note||A neomycin resistance cassette was inserted into exon 3 of the gene.|
|Mutations Made By|| |
Dr. Manfred Kopf, ETH Zurich
The strain is maintained by breeding homozygous mutants. The expected coat color from breeding is black.
When using the IL-5- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003175 in your Materials and Methods section.