C57BL/6-Il5^tm1Kopf/J

Stock number: 003175
Product name: IL-5-
Research areas: Cancer Research, Immunology, Inflammation and Autoimmunity Research

Description

These Il5 knock-out mice exhibit lack of eosinophilia response to infection and impaired CD5⁺ B-1 cell development.

Detailed description

Mice homozygous for the Il5^tm1Kopf targeted mutation are viable and fertile. Surprisingly, IL5 deficient mice show no defects in conventional B (B-2) cells, T cell dependent antibody production, or cytotoxic T cell responses. Eosinophilia response to infection is lacking in homozygous mutant mice. They also exhibit impaired CD5⁺ B-1 cell development.

Development

A targeting vector containing the neomycin resistance gene was used to disrupt exon 3. The construct was electroporated into C57BL/6J-derived BL/6-II embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6J.

Allele

Symbol: Il5^tm1Kopf
Name: targeted mutation 1, Manfred A Kopf
MGI accession ID: MGI:1861948
Generation method: Targeted
Attributes: Null/Knockout
Synonyms: IL-5-; IL-5^null
Marker symbol: Il5
Marker name: interleukin 5
Marker synonyms: EDF; Il-5; TRF
Chromosome: 11
Strain of origin: C57BL/6
Molecular note: A neomycin resistance cassette was inserted into exon 3 of the gene.