These IL-2Rγ-chain deficient mice are useful when studying NK cell development and innate immunity.
Dr. Warren J. Leonard, NHLBI, NIH
Genetic Background | Generation |
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N10+N4
|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Il2rg | interleukin 2 receptor, gamma chain |
Mice homozygous for the Il2rgtm1Wjl targeted mutation are viable and fertile. Mutant mice have hypoplastic thymuses with a 10-fold reduction in the absolute number of lymphocytes. They have a limited number of mature splenic B and T cells, lack NK cells and Peyer's patches, and development of gut-associated intraepithelial lymphocytes is diminished. In vitro studies show defects in NK activity, IL4-directed Ig class switching of thymocytes, and thymocyte mitogenic responses. Gene product (mRNA and protein) is not detected by Northern and western blot analysis of spleen or thymus tissue from hemizygous males.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector containing a neomycin resistance cassette was used to disrupt part of exon 3 and all of exons 4 through 8 of the Il2rg gene. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. The mice were then backcrossed to C57BL/6 for 10 generations by the Donating Investigator.
Allele Name | targeted mutation 1, Warren J Leonard |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | [KO]gammac; CD132-; gammac-; gc-; Il2rgtm1Wjll; IL2Rgammanull |
Gene Symbol and Name | Il2rg, interleukin 2 receptor, gamma chain |
Gene Synonym(s) | |
Site of Expression | Primarily lymphoid cells. |
Strain of Origin | 129S4/SvJae |
Chromosome | X |
Molecular Note | A neomycin resistance cassette replaced part of exon 3 and all of exons 4 - 8 of the gene, resulting in the loss of most of the extracellular domain and all of the transmembrane and cytoplasmic domains of the protein. |
Mutations Made By | Dr. Warren Leonard, NHLBI, NIH |
Il2rg is X-linked. When maintaining a live colony, homozygous females and hemizygous males may be bred together.
When using the IL2Rγnull mouse strain in a publication, please cite the originating article(s) and include JAX stock #003174 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
X linked - Heterozygous females and Wild-type Males for Il2rg<tm1Wjl> |
Frozen Mouse Embryo | B6.129S4-Il2rg<tm1Wjl>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Il2rg<tm1Wjl>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Il2rg<tm1Wjl>/J | $3373.50 |
Frozen Mouse Embryo | B6.129S4-Il2rg<tm1Wjl>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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