NOD.D2(B10)-Tg(Igh2^k3-83)1Nemz/Dvs

Stock number: 003172
Product name: Tol 1

Description

These Tol 1 mice express a transgene, on the NOD/Lt genetic background, containing a gene for IgM anti-H-2^k MHC class I antibody.

Detailed description

The phenotype of this transgene on a B10.D2nSnJ genetic background (Stock No. 003183) is as follows:

Hemizygous mice carry the immunoglobulin transgene Tg(Igh2k3-83)1Nemz. Results indicate that B cells specific for major histocompatibility complex class I antigen can be deleted if they encounter membrane-bound antigen at a post-bone-marrow stage of development. This deletion may be necessary to prevent organ-specific autoimmunity. The 3-83 transgenic only produce B-lymphocytes that make a single type of antibody with a defined specificity whose presence or absence can be easily monitored with several different reagents. Thus, they are invaluable to investigators who wish to determine how the development of a specific antibody specificity is genetically controlled. This is an important question in determining both how immune responses are generated against infectious agents, while normally preventing the development of antibodies against self-proteins which can lead to serious autoimmune diseases such as lupus. The 3-83 mice can also be used to dissect the antibody production role of B-lymphocytes from their antigen presenting cell functions.

Development

The construct for Tg(Igh2^k3-83)1Nemz was micro-injected into (DBA/2 x B10.D2) embryos and approximately four copies co-integrated in the same genetic locus. This transgene was imported into The Jackson Laboratory at greater than N12 on the B10.D2nSnJ background. There it was bred to NOD/Lt and hysterectomy-derived offspring were sent to the laboratory of Dr. David Serreze. He continued the backcross and in 2004 this strain, NOD.D2(B10)-Tg(Igh2^k3-83)1Nemz/Dvs, reached generation N17F3.

Allele

Symbol: Tg(Igh2^k3-83)1Nemz
Name: transgene insertion 3-83, David Nemazee
MGI accession ID: MGI:3055213
Generation method: Transgenic
Attributes: Inserted expressed sequence
Synonyms: 3-83; 3-83mudelta; 3-83mudelta-TG; mudelta TG
Marker symbol: Tg(Igh2^k3-83)1Nemz
Marker name: transgene insertion 3-83, David Nemazee
Marker synonyms: 3-83; 3-83mudelta; 3-83mudelta-TG; mudelta TG
Chromosome: UN
Strain of origin: DBA/2 x (C57BL/10 x DBA/2)F2
Expressed genes: Igh-Ia,immunoglobulin heavy chain Ia,mouse, laboratory
Molecular note: This immunoglobulin transgene (3-83 mu delta, anti-K-k,K-b) encodes antibody reactive with the membrane-bound H-2K(b) antigen.