These Tol 1 mice express a transgene, on the NOD/Lt genetic background, containing a gene for IgM anti-H-2k MHC class I antibody.
Read More +Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
The phenotype of this transgene on a B10.D2nSnJ genetic background (Stock No. 003183) is as follows:
Hemizygous mice carry the immunoglobulin transgene Tg(Igh2k3-83)1Nemz. Results indicate that B cells specific for major histocompatibility complex class I antigen can be deleted if they encounter membrane-bound antigen at a post-bone-marrow stage of development. This deletion may be necessary to prevent organ-specific autoimmunity. The 3-83 transgenic only produce B-lymphocytes that make a single type of antibody with a defined specificity whose presence or absence can be easily monitored with several different reagents. Thus, they are invaluable to investigators who wish to determine how the development of a specific antibody specificity is genetically controlled. This is an important question in determining both how immune responses are generated against infectious agents, while normally preventing the development of antibodies against self-proteins which can lead to serious autoimmune diseases such as lupus. The 3-83 mice can also be used to dissect the antibody production role of B-lymphocytes from their antigen presenting cell functions.
The construct for Tg(Igh2k3-83)1Nemz was micro-injected into (DBA/2 x B10.D2) embryos and approximately four copies co-integrated in the same genetic locus. This transgene was imported into The Jackson Laboratory at greater than N12 on the B10.D2nSnJ background. There it was bred to NOD/Lt and hysterectomy-derived offspring were sent to the laboratory of Dr. David Serreze. He continued the backcross and in 2004 this strain, NOD.D2(B10)-Tg(Igh2k3-83)1Nemz/Dvs, reached generation N17F3.
Expressed Gene | Igh-Ia, immunoglobulin heavy chain Ia, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 3-83, David Nemazee |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | 3-83; 3-83mudelta; 3-83mudelta-TG; mudelta TG |
Gene Symbol and Name | Tg(Igh2k3-83)1Nemz, transgene insertion 3-83, David Nemazee |
Gene Synonym(s) | |
Promoter | Igh-Ia, immunoglobulin heavy chain Ia, mouse, laboratory |
Expressed Gene | Igh-Ia, immunoglobulin heavy chain Ia, mouse, laboratory |
Strain of Origin | DBA/2 x (C57BL/10 x DBA/2)F2 |
Chromosome | UN |
Molecular Note | This immunoglobulin transgene (3-83 mu delta, anti-K-k,K-b) encodes antibody reactive with the membrane-bound H-2K(b) antigen. |
When using the Tol 1 mouse strain in a publication, please cite the originating article(s) and include JAX stock #003172 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous for Tg(Igh2<k>3-83)1Nemz |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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