FVB.Cg-Tg(Myh6-tTA)6Smbf/J

Stock number: 003170
Product name: 2.9alphatTA
Research areas: Research Tools

Description

This strain is currently unavailable due to replenishing of cryopreserved stocks.  Interested customers can register interest.

This strain may be useful for generating conditional expression of target genes in cardiac myocytes.

Detailed description

This strain expresses the tetracycline-controlled transactivator protein (tTA) under the regulatory control of the rat alpha myosin heavy chain promoter, which directs expression of tTA, specifically, in cardiac myocytes. When these Myh6-tTA (also called 2.9alphatTA or MHCAtTA) transgenic mice are mated to a second transgenic strain carrying a gene of interest coupled to a tetracycline-responsive promoter element (TRE), conditional expression of the target gene in cardiac myocytes may be controlled by the administration of tetracycline or doxycycline.

Development

A transgene in which ~ 2.9kb of 5' flanking sequence from the rat alpha myosin heavy chain gene drives expression of the tetracycline-controlled transactivator (tTA) was microinjected into C57BL/6xCBA F1 hybrid pronuclei. Subsequently, the strain was backcrossed to FVB/N for at least 8 generations.

Allele

Symbol: Tg(Myh6-tTA)6Smbf
Name: transgene insertion 6, Section of Myocardial Biology - Fishman Lab
MGI accession ID: MGI:2665551
Generation method: Transgenic
Attributes: Transactivator
Marker symbol: Tg(Myh6-tTA)6Smbf
Marker name: transgene insertion 6, Section of Myocardial Biology - Fishman Lab
Chromosome: UN
Strain of origin: (C57BL/6 x CBA)F1
Expressed genes: tTA,tetracycline-controlled transactivator,E. coli
Site of expression: Expresses tTA in cardiac myocytes.
Molecular note: The transgene consists of a rat Myh6 promoter and a tetracycline transactivator sequence. Transgene expression in cardiac tissue was confirmed by Northern blot analysis. 2.9 kb of rat Myh6 promoter sequences directs transgene expression in the heart starting at embryonic day E7.5.
General note: Transgenic line 6 showed the strongest cardiac transgene expression and was used for analysis.