Overview

Also Known As:2.9alphatTA

This strain is currently unavailable due to replenishing of cryopreserved stocks. Interested customers can register interest.

This strain may be useful for generating conditional expression of target genes in cardiac myocytes.

Donating Investigator

Dr. Glenn I. Fishman, NYU School of Medicine

Genetic overview

Genetic Background	Generation

Tg(Myh6-tTA)6Smbf

Allele Type
Transgenic (Transactivator)

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Research Applications

Research Tools

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Details

Important Note

This strain is homozygous for the retinal degeneration allele Pde6b^rd1.

Detailed Description

This strain expresses the tetracycline-controlled transactivator protein (tTA) under the regulatory control of the rat alpha myosin heavy chain promoter, which directs expression of tTA, specifically, in cardiac myocytes. When these Myh6-tTA (also called 2.9alphatTA or MHCAtTA) transgenic mice are mated to a second transgenic strain carrying a gene of interest coupled to a tetracycline-responsive promoter element (TRE), conditional expression of the target gene in cardiac myocytes may be controlled by the administration of tetracycline or doxycycline.

Development

A transgene in which ~ 2.9kb of 5' flanking sequence from the rat alpha myosin heavy chain gene drives expression of the tetracycline-controlled transactivator (tTA) was microinjected into C57BL/6xCBA F1 hybrid pronuclei. Subsequently, the strain was backcrossed to FVB/N for at least 8 generations.

Expression Data

Expressed Gene	tTA, tetracycline-controlled transactivator, E. coli
Site of Expression	Expresses tTA in cardiac myocytes.

Control Suggestions

Noncarrier
001800 FVB/NJ

Additional Information

Considerations for Choosing Controls

Selected References

Yu Z; Redfern CS; Fishman GI. 1996. Conditional transgene expression in the heart. Circ Res 79(4):691-7PubMed: 8831492 MGI: J:82692

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Genetics

Tg(Myh6-tTA)6Smbf

Allele Symbol: Tg(Myh6-tTA)6Smbf

Allele Name	transgene insertion 6, Section of Myocardial Biology - Fishman Lab
Allele Type	Transgenic (Transactivator)
Allele Synonym(s)	2.9alphatTA; alphaMHC-tTA; alphaMtTA; Tg(MHCAtTA)6Smbf
Gene Symbol and Name	Tg(Myh6-tTA)6Smbf, transgene insertion 6, Section of Myocardial Biology - Fishman Lab
Gene Synonym(s)
Promoter	Myh6, myosin heavy chain 6, rat
Expressed Gene	tTA, tetracycline-controlled transactivator, E. coli
Site of Expression	Expresses tTA in cardiac myocytes.
Strain of Origin	(C57BL/6 x CBA)F1
Chromosome	UN
General Note	Transgenic line 6 showed the strongest cardiac transgene expression and was used for analysis.
Molecular Note	The transgene consists of a rat Myh6 promoter and a tetracycline transactivator sequence. Transgene expression in cardiac tissue was confirmed by Northern blot analysis. 2.9 kb of rat Myh6 promoter sequences directs transgene expression in the heart starting at embryonic day E7.5.
Mutations Made By	Dr. Glenn Fishman, NYU School of Medicine

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Cardiovascular Research
- Tet Expression Systems
  - tTA/rtTA Expressing Strains

Mammalian Phenotype Terms by Genotype

References

Yu Z; Redfern CS; Fishman GI. 1996. Conditional transgene expression in the heart. Circ Res 79(4):691-7PubMed: 8831492 MGI: J:82692

Additional References

Bowman JC; Steinberg SF; Jiang T; Geenen DL; Fishman GI; Buttrick PM. 1997. Expression of protein kinase C beta in the heart causes hypertrophy in adult mice and sudden death in neonates. J Clin Invest 100(9):2189-95PubMed: 9410895 MGI: J:43986
Ward NL; Van Slyke P; Sturk C; Cruz M; Dumont DJ. 2004. Angiopoietin 1 expression levels in the myocardium direct coronary vessel development. Dev Dyn 229(3):500-9PubMed: 14991706 MGI: J:88848

Additional - Tg(Myh6-tTA)6Smbf related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Generic rtTA-Alternate 1
- Probe:Generic tTA/rtTA
- QPCR:Tg(tTA)
- Standard PCR:Tg(tTA)
- Standard PCR:Tg(tTA)
- Standard PCR:Generic tTA
- Probe:Generic rtTA-Alternate 1
- Standard PCR:Tg(Myh6-tTA)6Smbf-Alternate 2
- Genotyping resources and troubleshooting
Breeding Considerations

When mating to tet responder animals, doxycycline should be administered to the female to avoid an embryonic lethal phenotype. This strain is fed normal chow diet. Expected coat color from breeding is "Albino."
- Additional Breeding and Husbandry Support
Appearance
- albino
  Related Genotype: Tyr^c/Tyr^c
Citation
When using the 2.9alphatTA mouse strain in a publication, please cite the originating article(s) and include JAX stock #003170 in your Materials and Methods section.

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Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:2.9alphatTA

Donating Investigator

Genetic overview

Research Applications

Important Note

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Tg(Myh6-tTA)6Smbf

Allele Symbol: Tg(Myh6-tTA)6Smbf

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional References

Additional - Tg(Myh6-tTA)6Smbf related

Genotyping Protocols

Breeding Considerations

Appearance

Citation

Strain Interest Registration

Contact Information

Interest

Terms Of Use

Additional Use Restrictions Apply

Licensing Information