This strain may be useful for generating conditional expression of target genes in cardiac myocytes.
Dr. Glenn I. Fishman, NYU School of Medicine
Genetic Background | Generation |
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Allele Type |
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Transgenic (Transactivator) |
This strain is homozygous for the retinal degeneration allele Pde6brd1.
This strain expresses the tetracycline-controlled transactivator protein (tTA) under the regulatory control of the rat alpha myosin heavy chain promoter, which directs expression of tTA, specifically, in cardiac myocytes. When these Myh6-tTA (also called 2.9alphatTA or MHCAtTA) transgenic mice are mated to a second transgenic strain carrying a gene of interest coupled to a tetracycline-responsive promoter element (TRE), conditional expression of the target gene in cardiac myocytes may be controlled by the administration of tetracycline or doxycycline.
A transgene in which ~ 2.9kb of 5' flanking sequence from the rat alpha myosin heavy chain gene drives expression of the tetracycline-controlled transactivator (tTA) was microinjected into C57BL/6xCBA F1 hybrid pronuclei. Subsequently, the strain was backcrossed to FVB/N for at least 8 generations.
Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli |
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Site of Expression | Expresses tTA in cardiac myocytes. |
Allele Name | transgene insertion 6, Section of Myocardial Biology - Fishman Lab |
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Allele Type | Transgenic (Transactivator) |
Allele Synonym(s) | 2.9alphatTA; alphaMHC-tTA; alphaMtTA; Tg(MHCAtTA)6Smbf |
Gene Symbol and Name | Tg(Myh6-tTA)6Smbf, transgene insertion 6, Section of Myocardial Biology - Fishman Lab |
Gene Synonym(s) | |
Promoter | Myh6, myosin heavy chain 6, rat |
Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli |
Site of Expression | Expresses tTA in cardiac myocytes. |
Strain of Origin | (C57BL/6 x CBA)F1 |
Chromosome | UN |
General Note | Transgenic line 6 showed the strongest cardiac transgene expression and was used for analysis. |
Molecular Note | The transgene consists of a rat Myh6 promoter and a tetracycline transactivator sequence. Transgene expression in cardiac tissue was confirmed by Northern blot analysis. 2.9 kb of rat Myh6 promoter sequences directs transgene expression in the heart starting at embryonic day E7.5. |
Mutations Made By | Dr. Glenn Fishman, NYU School of Medicine |
When mating to tet responder animals, doxycycline should be administered to the female to avoid an embryonic lethal phenotype. This strain is fed normal chow diet. Expected coat color from breeding is "Albino."
When using the 2.9alphatTA mouse strain in a publication, please cite the originating article(s) and include JAX stock #003170 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non Carrier for Tg(Myh6-tTA)6Smbf |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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