These Il2rg knock-out mice exhibit hypoplastic thymuses with a reduction in lymphocyte number and diminishment of gut-associated intraepithelial lymphocytes.
Dr. Warren J. Leonard, NHLBI, NIH
Il2rg is X-linked. Homozygous female and hemizygous male mice carrying the Il2rgtm1Wjl targeted mutation are viable and fertile. Mutant mice have hypoplastic thymuses with a 10-fold reduction in the absolute number of lymphocytes. They have a limited number of mature splenic B and T cells, lack NK cells and Peyer's patches, and development of gut-associated intraepithelial lymphocytes is diminished. In vitro studies show defects in NK activity, IL4-directed Ig class switching of thymocytes, and thymocyte mitogenic responses. Gene product (mRNA and protein) is not detected by Northern and western blot analysis of spleen or thymus tissue from hemizygous males.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector containing neomycin resistance cassette was used to disrupt part of exon 3 and all of exons 4 through 8 of the Il2rg gene. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. The mice were then backcrossed to BALB/c for 10 generations by the Donating Investigator.
|Allele Name||targeted mutation 1, Warren J Leonard|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||[KO]gammac; CD132-; gammac-; gc-; Il2rgtm1Wjll; IL2Rgammanull|
|Gene Symbol and Name||Il2rg, interleukin 2 receptor, gamma chain|
|Site of Expression||Primarily lymphoid cells.|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A neomycin resistance cassette replaced part of exon 3 and all of exons 4 - 8 of the gene, resulting in the loss of most of the extracellular domain and all of the transmembrane and cytoplasmic domains of the protein.|
|Mutations Made By|| |
Dr. Warren Leonard, NHLBI, NIH
Il2rg is X-linked. When maintaining a live colony, homozygous females and hemizygous males may be bred together.
When using the IL2Rγnull mouse strain in a publication, please cite the originating article(s) and include JAX stock #003169 in your Materials and Methods section.