These DBH-lacZ mice express a transgene containing beta-galactosidase driven by dopamine beta hydroxylase promotor.
Dr. Raj P. Kapur, University of Washington Medical Center
Genetic Background | Generation |
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000664 C57BL/6J |
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Allele Type |
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Transgenic (Reporter) |
Transgenic mice carry a beta-galactosidase reporter gene driven by dopamine beta hydroxylase promotor. LacZ expression is seen in neurons of the locus ceruleus and other classic noradrenergic brain stem nuclei, sympathetic ganglion neurons, and adrenal chromaffin cells. LacZ expression is also observed in neurons of the enteric system, the retina, some sensory and all cranial parasympathetic ganglia, and some diencephalic and telencephalic brain nuclei.
A DBH-lac fusion gene was made and tested intransgenic mice. A fragment of the human DBH gene, extending from an Smal site 5.8 kb upstream from the transcription start to an Hpall site 41 bp into the 5' untranslated region of exon 1, was fused to the cloned E. coli lacZ gene of placF. This strain is identified as line DBH-nlacZ(2860-8)in the primary publication.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | CNS expression. Specifically, neurons of the locus ceruleus and other classic noradrenergic brain stem nuclei, sympathetic ganglion neurons, and adrenal chromaffin cells. Also in neurons of the enteric system, the retina, some sensory and all cranial parasympathetic ganglia, and some diencephalic and telencephalic brain nuclei. |
Allele Name | transgene insertion 8, Raj P Kapur |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | DBH-lacZ; DBH-nlacZ(2860-8) |
Gene Symbol and Name | Tg(DBHn-lacZ)8Rpk, transgene insertion 8, Raj P Kapur |
Gene Synonym(s) | |
Promoter | DBH, dopamine beta-hydroxylase, human |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | CNS expression. Specifically, neurons of the locus ceruleus and other classic noradrenergic brain stem nuclei, sympathetic ganglion neurons, and adrenal chromaffin cells. Also in neurons of the enteric system, the retina, some sensory and all cranial parasympathetic ganglia, and some diencephalic and telencephalic brain nuclei. |
Strain of Origin | C57BL/6 x SJL |
Chromosome | UN |
General Note | This line was generated from founder 2860-8. LacZ expression is seen in neurons of the locus ceruleus and other classic noradrenergic brain stem nuclei, sympathetic ganglion neurons, and adrenal chromaffin cells. LacZ expression is also observed in neurons of the enteric system, the retina, some sensory and all cranial parasympathetic ganglia, and some diencephalic and telencephalic brain nuclei. |
Molecular Note | A fragment of the human dopamine beta hydroxylase (DBH) gene, extending from a Sma1 site 5.8 kb upstream from the transcription start to an HpaII site 41 bp into the 5' untranslated region of exon 1, was fused to a bacterial beta-galactosidase reporter gene. |
This strain originated on a B6;SJL background and is maintained by mating heterozygotes to C57BL/6J. Reproduction is excellent. Coat color expected from breeding:Black
When using the DBH-lacZ mouse strain in a publication, please cite the originating article(s) and include JAX stock #003139 in your Materials and Methods section.
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Hemizygous or Non carrier for Tg(DBHn-lacZ)8Rpk |
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