B6.Cg-Tg(DBHn-lacZ)8Rpk/J

Stock number: 003139
Product name: DBH-lacZ
Research areas: Neurobiology Research, Research Tools

Description

These DBH-lacZ mice express a transgene containing beta-galactosidase driven by dopamine beta hydroxylase promotor.

Detailed description

Transgenic mice carry a beta-galactosidase reporter gene driven by dopamine beta hydroxylase promotor. LacZ expression is seen in neurons of the locus ceruleus and other classic noradrenergic brain stem nuclei, sympathetic ganglion neurons, and adrenal chromaffin cells. LacZ expression is also observed in neurons of the enteric system, the retina, some sensory and all cranial parasympathetic ganglia, and some diencephalic and telencephalic brain nuclei.

Development

A DBH-lac fusion gene was made and tested intransgenic mice. A fragment of the human DBH gene, extending from an Smal site 5.8 kb upstream from the transcription start to an Hpall site 41 bp into the 5' untranslated region of exon 1, was fused to the cloned E. coli lacZ gene of placF. This strain is identified as line DBH-nlacZ(2860-8)in the primary publication.

Allele

Symbol: Tg(DBHn-lacZ)8Rpk
Name: transgene insertion 8, Raj P Kapur
MGI accession ID: MGI:3055106
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(DBHn-lacZ)8Rpk
Marker name: transgene insertion 8, Raj P Kapur
Chromosome: UN
Strain of origin: C57BL/6 x SJL
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: CNS expression. Specifically, neurons of the locus ceruleus and other classic noradrenergic brain stem nuclei, sympathetic ganglion neurons, and adrenal chromaffin cells. Also in neurons of the enteric system, the retina, some sensory and all cranial parasympathetic ganglia, and some diencephalic and telencephalic brain nuclei.
Molecular note: A fragment of the human dopamine beta hydroxylase (DBH) gene, extending from a Sma1 site 5.8 kb upstream from the transcription start to an HpaII site 41 bp into the 5' untranslated region of exon 1, was fused to a bacterial beta-galactosidase reporter gene.
General note: This line was generated from founder 2860-8.

LacZ expression is seen in neurons of the locus ceruleus and other classic noradrenergic brain stem nuclei, sympathetic ganglion neurons, and adrenal chromaffin cells. LacZ expression is also observed in neurons of the enteric system, the retina, some sensory and all cranial parasympathetic ganglia, and some diencephalic and telencephalic brain nuclei.