These Cdkn1b knock-out mice exhibit an enlarged thymus and spleen and are susceptible to the development of adenomas upon induction by gamma irradiation or chemical carcinogens.
Dr. Matthew Fero, Fred Hutchinson Cancer Research Center
Mice deficient in p27kip are viable, but are larger than normal littermates, with increased cellularity of all tissues. The thymus and spleen are particularly enlarged. Homozygous mutant, Cdkn1b-null adult mice have a shortened lifespan due to the growth of benign intermediate lobe pituitary tumors. Female mice are infertile, with a follicular phase ovulatory block. Large doses of exogenous gonadotropin induce ovulation, but both implantation and intrauterine embryonic development is impaired. The mice demonstrate haploid-insufficient susceptibility to the development of adenomas in the pituitary, intestine and lung following exposure to gamma irradiation or chemical carcinogens.
|Allele Name||targeted mutation 1, Matthew Fero|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Cdkn1btm1Mlf; targeted mutation 1, Matthew Fero|
|Gene Symbol and Name||Cdkn1b, cyclin-dependent kinase inhibitor 1B|
|Gene Synonym(s)||expressed sequence AA408329; p27; P27KIP1; expressed sequence AI843786; AA408329; AI843786; KIP1; Cdki1b; CDKN4; MEN1B; MEN4; p27Kip1; Kip1|
|Strain of Origin||129S4/SvJaeSor|
|Molecular Note||Replacement of the entire coding region of the Cdkn1b gene with a neomycin cassette.|
|Mutations Made By|| |
Dr. Matthew Fero, Fred Hutchinson Cancer Research Center
When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to 129S1/SvImJ inbred mice (Stock No. 002448). Homozygotes have a shortened lifespan due to the growth of benign intermediate lobe pituitary tumors. Homozygous female mice are infertile.
When using the p27- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003122 in your Materials and Methods section.
|Heterozygous or Wild-type for Cdkn1b<tm1Mlf>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
|Frozen Mouse Embryo||$2,595.00 per straw or vial|
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