These Cdkn1b knock-out mice exhibit an enlarged thymus and spleen and are susceptible to the development of adenomas upon induction by gamma irradiation or chemical carcinogens.
Dr. Matthew Fero, Fred Hutchinson Cancer Research Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Cdkn1b | cyclin-dependent kinase inhibitor 1B |
Mice deficient in p27kip are viable, but are larger than normal littermates, with increased cellularity of all tissues. The thymus and spleen are particularly enlarged. Homozygous mutant, Cdkn1b-null adult mice have a shortened lifespan due to the growth of benign intermediate lobe pituitary tumors. Female mice are infertile, with a follicular phase ovulatory block. Large doses of exogenous gonadotropin induce ovulation, but both implantation and intrauterine embryonic development is impaired. The mice demonstrate haploid-insufficient susceptibility to the development of adenomas in the pituitary, intestine and lung following exposure to gamma irradiation or chemical carcinogens.
Allele Name | targeted mutation 1, Matthew Fero |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Cdkln1btm1Mlf; p27-; p27Kip1; p27Kip1-; p27kip1-null |
Gene Symbol and Name | Cdkn1b, cyclin-dependent kinase inhibitor 1B |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJaeSor |
Chromosome | 6 |
Molecular Note | Replacement of the entire coding region of the Cdkn1b gene with a neomycin cassette. |
Mutations Made By | Dr. Matthew Fero, Fred Hutchinson Cancer Research Center |
When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to 129S1/SvImJ inbred mice (Stock No. 002448). Homozygotes have a shortened lifespan due to the growth of benign intermediate lobe pituitary tumors. Homozygous female mice are infertile.
When using the p27- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003122 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Cdkn1b<tm1Mlf> |
Frozen Mouse Embryo | 129-Cdkn1b<tm1Mlf>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | 129-Cdkn1b<tm1Mlf>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | 129-Cdkn1b<tm1Mlf>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | 129-Cdkn1b<tm1Mlf>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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