129S-Sst^tm1Ute/J

Stock number: 003117
Product name: SRIF KO
Research areas: Cancer Research, Endocrine Deficiency Research, Immunology, Inflammation and Autoimmunity Research, Neurobiology Research

Description

These Sst knock-out mice exhibit absence of somatostatin in the adult cerebellum. They are suitable for use in applications related to the study of neurotransmission and development.

Detailed description

Mutant mice are healthy, fertile and of normal body size despite having three times the plasma growth hormone levels than wildtype littermates. Somatostatin is virtually absent in the adult cerebellum but is expressed during development. There is impaired motor learning and motor coordination as demonstrated in accelerating rotorod testing.

Development

An insertion of selection cassette into the preprosomatostatin locus, thereby deleting 30 basepairs of the 3' end of exon 1 and 39 basepairs of the 5' end of intron 1.

Allele

Symbol: Sst^tm1Ute
Name: targeted mutation 1, Ute Hochgeschwender
MGI accession ID: MGI:1888381
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Sst
Marker name: somatostatin
Chromosome: 16
Strain of origin: 129S4/SvJae
Molecular note: An SV40-tk-neo cassette was inserted, thereby deleting the last 30 bp of exon 1 and the first 39 basepairs of inton 1. Successful recombination was shown by Southern blot. Northern blot indicated lack of transcript and radioimmunoassay demonstrated the lack of protein from homozygous mutants.