These Sst knock-out mice exhibit absence of somatostatin in the adult cerebellum. They are suitable for use in applications related to the study of neurotransmission and development.
Ute Hochgeschwender, Central Michigan University
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Sst | somatostatin |
Mutant mice are healthy, fertile and of normal body size despite having three times the plasma growth hormone levels than wildtype littermates. Somatostatin is virtually absent in the adult cerebellum but is expressed during development. There is impaired motor learning and motor coordination as demonstrated in accelerating rotorod testing.
An insertion of selection cassette into the preprosomatostatin locus, thereby deleting 30 basepairs of the 3' end of exon 1 and 39 basepairs of the 5' end of intron 1.
Allele Name | targeted mutation 1, Ute Hochgeschwender |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Srif-; Sst-; Sst KO |
Gene Symbol and Name | Sst, somatostatin |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 16 |
Molecular Note | An SV40-tk-neo cassette was inserted, thereby deleting the last 30 bp of exon 1 and the first 39 basepairs of inton 1. Successful recombination was shown by Southern blot. Northern blot indicated lack of transcript and radioimmunoassay demonstrated the lack of protein from homozygous mutants. |
Mutations Made By | Ute Hochgeschwender, Central Michigan University |
The originating investigator maintains the strain by mating heterozygous siblings. Litter size averages 6 pups. Homozygous animals are viable and fertile. Expected coat color from breeding:White Bellied Agouti
When using the SRIF KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #003117 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Sst<tm1Ute> |
Frozen Mouse Embryo | 129S-Sst<tm1Ute>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | 129S-Sst<tm1Ute>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | 129S-Sst<tm1Ute>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | 129S-Sst<tm1Ute>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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