STOCK Ptch1^tm1Mps/J

Stock number: 003081
Product name: ptc-lacz
Research areas: Cancer Research, Developmental Biology Research

Description

In this reporter targeted mutant strain a promoterless lacZ-neo fusion gene was inserted at the start codon and deleted a portion of exon 1 and all of exon 2 of the Ptch1 (patched homolog 1) gene. Expression of the inserted lacZ gene served as an accurate reporter in heterozygotes but was found to be derepressed in homozygous mutant mice.

Detailed description

Mice homozygous for the targeted mutation die during embryogenesis and are found to have open and overgrown neural tubes. Heterozygous patched mice are larger than wild-type littermates and have a low incidence of hindlimb defects. Some heterozygotes develop brain tumors beginning around 5 weeks of age. Heterozygotes express lacZ in a pattern mimicking endogenous gene expression pattern. Homozygous embryos display derepressed lacZ expression starting at embryonic day 8.0.

Development

A targeting vector, KO1, containing sequence encoding nuclear localized beta-galactosidase (lacZ), neomycin resistance gene, mouse protamine 1 (mP1) intron, and a polyadenylation site was electroporated into 129-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric male mice were then mated to B6D2F1 females.

Allele

Symbol: Ptch1^tm1Mps
Name: targeted mutation 1, Matthew P Scott
MGI accession ID: MGI:1857447
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Ptch1
Marker name: patched 1
Chromosome: 13
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Expressed genes: lacZ,beta-galactosidase,E. coli
Molecular note: A construct containing a promoterless lacZ-neo fusion gene was inserted at the start codon and deleted a portion of exon 1 and all of exon 2, which encoded the first transmembrane domain. Expression of the inserted lacZ gene served as an accurate reporter in heterozygotes but was found to be derepressed in homozygous mutant mice.