In this reporter targeted mutant strain a promoterless lacZ-neo fusion gene was inserted at the start codon and deleted a portion of exon 1 and all of exon 2 of the Ptch1 (patched homolog 1) gene. Expression of the inserted lacZ gene served as an accurate reporter in heterozygotes but was found to be derepressed in homozygous mutant mice.
Dr. Matthew Scott, Stanford University School of MedicineRead More +
This strain is segregating the coat color allele dilute (Myo5ad). Mice can be either black or dilute grey.
Mice homozygous for the targeted mutation die during embryogenesis and are found to have open and overgrown neural tubes. Heterozygous patched mice are larger than wild-type littermates and have a low incidence of hindlimb defects. Some heterozygotes develop brain tumors beginning around 5 weeks of age. Heterozygotes express lacZ in a pattern mimicking endogenous gene expression pattern. Homozygous embryos display derepressed lacZ expression starting at embryonic day 8.0.
A targeting vector, KO1, containing sequence encoding nuclear localized beta-galactosidase (lacZ), neomycin resistance gene, mouse protamine 1 (mP1) intron, and a polyadenylation site was electroporated into 129-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric male mice were then mated to B6D2F1 females.
|Expressed Gene||lacZ, beta-galactosidase, E. coli|
|Site of Expression|
|Allele Name||targeted mutation 1, Matthew P Scott|
|Allele Type||Targeted (Null/Knockout, Reporter)|
|Allele Synonym(s)||LacZ/Ptc; Ptc1-lacZ; Ptc1-; Ptc1lzki; PtclacZ; Ptcn; Ptch-; Ptch1-; Ptch1-lacZ; PtchKO1; PtchlacZ; ptc-; ptc-lacZ|
|Gene Symbol and Name||Ptch1, patched 1|
|Gene Synonym(s)||A230106A15Rik; A230106A15Rik; BCNS; HPE7; NBCCS; PTC; PTC1; PTCH; PTCH11; Patched 1; Ptc; Ptc1; Ptch; Ptch2; RIKEN cDNA A230106A15 gene; mes; mesenchymal dysplasia|
|Expressed Gene||lacZ, beta-galactosidase, E. coli|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl<+>|
|Molecular Note||A construct containing a promoterless lacZ-neo fusion gene was inserted at the start codon and deleted a portion of exon 1 and all of exon 2, which encoded the first transmembrane domain. Expression of the inserted lacZ gene served as an accurate reporter in heterozygotes but was found to be derepressed in homozygous mutant mice.|
|Mutations Made By|| |
Dr. Matthew Scott, Stanford University School of Medicine
When maintaining a live colony, heterozygous mice are bred with wildtype mice from the colony. Homozygotes die in utero. Expected coat color from breeding is Black,Gray.
|Please inquire about possible genotypes.|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
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