These Emx1 knock-out mice exhibit a lack of most or all of their corpus callosum. They are suitable for use in applications related to the study of cerebral cortex development.
Dr. John Rubenstein, Univ of Calif, San Francisco
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Emx1 | empty spiracles homeobox 1 |
Mice homozygous for the Emx1 tm1Jlr targeted mutation are viable and fertile. They show no obvious morphological or behavioral abnormalities. However, all homozygous mice lack most or all of their corpus callosum, the principle fiber tract that connects the left and right cerebral hemispheres. Heterozygotes show partial penetrance for the corpus callosum abnormality. The cerebral cortex appears normal in both heterozygous and homozygous mice.
A targeting vector containing PGKneo gene cassette was used to disrupt exon 1 and part of intron 1, encoding the N-terminus. The construct was electroporated into 129X1/SvJ derived JM1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to generate homozygotes.
Allele Name | targeted mutation 1, John Rubenstein |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Emx-1-; Emx1null |
Gene Symbol and Name | Emx1, empty spiracles homeobox 1 |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 6 |
Molecular Note | Insertion of a PGK-neomycin resistance cassette replaced exon 1 and part of intron 1 of the Emx1 gene. Northern analysis of E14.5 embryos did not detect Emx1 transcript in homozygous mutant mice. The authors predict that the mutant allele would produce a truncated protein that lacks the N-terminus. |
Mutations Made By | Dr. John Rubenstein, Univ of Calif, San Francisco |
This strain is maintained by homozyous sibling matings. Expected coat color from breeding:Black
When using the Emx-1- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003080 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Homozygous for Emx1<tm1Jlr> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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