B6;129X1-Emx1^tm1Jlr/J

Stock number: 003080
Product name: Emx-1-
Research areas: Developmental Biology Research, Neurobiology Research

Description

These Emx1 knock-out mice exhibit a lack of most or all of their corpus callosum. They are suitable for use in applications related to the study of cerebral cortex development.

Detailed description

Mice homozygous for the Emx1 ^tm1Jlr targeted mutation are viable and fertile. They show no obvious morphological or behavioral abnormalities. However, all homozygous mice lack most or all of their corpus callosum, the principle fiber tract that connects the left and right cerebral hemispheres. Heterozygotes show partial penetrance for the corpus callosum abnormality. The cerebral cortex appears normal in both heterozygous and homozygous mice.

Development

A targeting vector containing PGKneo gene cassette was used to disrupt exon 1 and part of intron 1, encoding the N-terminus. The construct was electroporated into 129X1/SvJ derived JM1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to generate homozygotes.

Allele

Symbol: Emx1^tm1Jlr
Name: targeted mutation 1, John Rubenstein
MGI accession ID: MGI:1857433
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Emx1
Marker name: empty spiracles homeobox 1
Chromosome: 6
Strain of origin: 129X1/SvJ
Molecular note: Insertion of a PGK-neomycin resistance cassette replaced exon 1 and part of intron 1 of the Emx1 gene. Northern analysis of E14.5 embryos did not detect Emx1 transcript in homozygous mutant mice. The authors predict that the mutant allele would produce a truncated protein that lacks the N-terminus.