NMRI/Gat-Tg(MGMT)3Bec/J

Stock number: 003076
Product name: CkMGMT
Research areas: Cancer Research, Research Tools

Description

These CkMGMT mice express a transgene containing the human DNA repair protein MGMT (O6-methylguanine-DNA methyltransferase).

Detailed description

This strain carries a transgene that directs skin-targeted overexpression of the human DNA repair protein MGMT (O6-methylguanine-DNA methyltransferase). The transgenic strain exhibits a significantly reduced incidence of alkylation-induced skin tumors. It may be used (1) to investigate the role of the MGMT DNA repair enzyme in protecting cells from premutagenic and precarcinogenic O6-alkylguanine lesions, (2) as an indicator system for O6-alkylguanine generating carcinogens (e.g. N-nitrosamines, N-nitrosoureas) and (3) to explore the pathophysiological significance of these lesions for various end points. Moreover, skin tumors induced on these transgenic mice may be used as a model for chemotherapy resistant tumors expressing high levels of MGMT.

Allele

Symbol: Tg(MGMT)3Bec
Name: transgene insertion 3, Klaus Becker
MGI accession ID: MGI:3055098
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(MGMT)3Bec
Marker name: transgene insertion 3, Klaus Becker
Chromosome: UN
Strain of origin: NMRI
Expressed genes: MGMT,O-6-methylguanine-DNA methyltransferase,human
Molecular note: Transgene contains a cytokeratin (Ck) promoter that directs skin targeted overexpression of the human DNA repair protein MGMT (O6-methylguanine-DNA methyltransferase).