B6.NOD-(Csf2-D11Mit42)/J

Stock number: 003065
Product name: B6.NOD Idd4
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research

Description

These congenic mice carry a chromosomal segment from Chromosome 11 bounded by the colony stimulating factor 2 (Csf2) and an Mit marker and includes the insulin dependent diabetes susceptibility locus 4 (Idd4).

Detailed description

This strain is congenic for a 42 cM segment of Chr 11 extending from Csf2 (colony stimulating factor 2, granulocyte-macrophage) through D11Mit42 that includes the insulin dependent diabetes susceptibility locus Idd4.The name given this segment in the primary reference is c11. Upon histological examination of the pancreas, the incidence and severity of periinsulitis (perivascular, periductal and/or periislet infiltration by mononuclear cells) in B6.NOD-(Csf2-D11Mit42) mice were found to be similar to those in C57BL/6 controls.

Development

Genomic segments found in earlier linkage studies to include diabetogenic loci were transferred in the laboratory of E.K. Wakeland from NOD/Uf to C57BL/6 by six successive backcrosses (to N7). Sibs of each lineage were then intercrossed to generate mice homozygous for each segment. Microsatellite analysis was used to type mice for loci in the regions of interest.

Allele

Symbol: Idd4^NOD
Name: NOD
MGI accession ID: MGI:3036664
Generation method: QTL
Marker symbol: Idd4
Marker name: insulin dependent diabetes susceptibility 4
Marker synonyms: Idd-4
Chromosome: 11
Strain of origin: NOD
Molecular note: This allele confers susceptibility to insulin dependent diabetes compared to C57BL/10.
General note: This allele also confers decreased hepatic iNKT cell numbers on a C57BL/6 background.

Allele

MGI accession ID: MGI:89126
Marker symbol: D11Mit42
Marker name: DNA segment, Chr 11, Massachusetts Institute of Technology 42
Chromosome: 11

Allele

MGI accession ID: MGI:1339752
Marker symbol: Csf2
Marker name: colony stimulating factor 2 (granulocyte-macrophage)
Marker synonyms: CSF; Csfgm; GMCSF; Gm-csf; MGI-IGM
Chromosome: 11