B6.NOD-(D6Mit54-D6Mit14)/J

Stock number: 003063
Product name: B6.NOD Idd6
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research, Metabolism Research

Description

These congenic mice carry a chromosomal segment from Chromosome 6 bounded by Mit markers and includes the insulin dependent diabetes susceptibility locus 6 (Idd6).

Detailed description

This strain is congenic for a 24 cM segment of Chr 6 extending from D6Mit54through D6Mit14that includes the insulin dependent diabetes susceptibility locus Idd6.The name given this segment in the primary reference is c6. Upon histologic examination of the pancreas, the incidence and severity of periinsulitis (perivascular, periductal and/or periislet infiltration by mononuclear cells) of B6.NOD-(D6Mit54-D6Mit14) mice were found to be similar to those of C57BL/6 controls.

Development

Genomic segments found in earlier linkage studies to include diabetogenic loci were transferred in the laboratory of E.K. Wakeland from NOD/Uf to C57BL/6 by six successive backcrosses (to N7). Sibs of each lineage were then intercrossed to generate mice homozygous for each segment. Microsatellite analysis was used to type mice for loci in the regions of interest.

Allele

Symbol: Idd6^NOD
Name: NOD
MGI accession ID: MGI:3036667
Generation method: QTL
Marker symbol: Idd6
Marker name: insulin dependent diabetes susceptibility 6
Chromosome: 6
Strain of origin: NOD
Molecular note: This allele confers impaired thymocyte proliferation compared to C57BL/6.
General note: This allele also confers defective IFN-gamma response in NK cells on a C57BL/6 genetic background (J:85823).

Allele

MGI accession ID: MGI:93686
Marker symbol: D6Mit54
Marker name: DNA segment, Chr 6, Massachusetts Institute of Technology 54
Chromosome: 6

Allele

MGI accession ID: MGI:93568
Marker symbol: D6Mit14
Marker name: DNA segment, Chr 6, Massachusetts Institute of Technology 14
Chromosome: 6