C.129S1-Il12rb1^tm1Jm/J

Stock number: 003017
Product name: IL-12Rβ1-
Research areas: Cancer Research, Immunology, Inflammation and Autoimmunity Research

Description

These mice carry a spontaneous mutation at the Il12rb1 locus characterized by impairment to produce interferon-gamma in response to endotoxin administration in vivo.

Detailed description

Mice homozygous for the Il12rb^tm1Jm targeted mutation are viable and fertile. Con A-activated splenocytes from homozygous mutant mice express only low affinity IL12-binding sites and fail to proliferate or produce interferon gamma in response to IL12. In addition, NK lytic activity in response to IL12 is defective. In vivo immune responses to endotoxin are also impaired.

Allele

Symbol: Il12rb1^tm1Jm
Name: targeted mutation 1, Jeanne Magram
MGI accession ID: MGI:1857440
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Il12rb1
Marker name: interleukin 12 receptor, beta 1
Chromosome: 8
Strain of origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Molecular note: A neomycin resistance cassette replaced exons 1 to 3 of the gene, deleting the ATG translation initiation codon and causing a frame-shift in the translation reading frame.