These mice carry a spontaneous mutation at the Il12rb1 locus characterized by impairment to produce interferon-gamma in response to endotoxin administration in vivo.
Dr. Jeanne Magram, Celsius Therapeutics
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Il12rb1 | interleukin 12 receptor, beta 1 |
Mice homozygous for the Il12rbtm1Jm targeted mutation are viable and fertile. Con A-activated splenocytes from homozygous mutant mice express only low affinity IL12-binding sites and fail to proliferate or produce interferon gamma in response to IL12. In addition, NK lytic activity in response to IL12 is defective. In vivo immune responses to endotoxin are also impaired.
Allele Name | targeted mutation 1, Jeanne Magram |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | IL-12Rbeta1-; IL-12Rbeta1KO |
Gene Symbol and Name | Il12rb1, interleukin 12 receptor, beta 1 |
Gene Synonym(s) | |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 8 |
Molecular Note | A neomycin resistance cassette replaced exons 1 to 3 of the gene, deleting the ATG translation initiation codon and causing a frame-shift in the translation reading frame. |
Mutations Made By | Dr. Jeanne Magram, Celsius Therapeutics |
When maintaining a live colony, homozygous mice may be bred together.
When using the IL-12Rβ1- mouse strain in a publication, please cite the originating article(s) and include JAX stock #003017 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Il12rb1<tm1Jm> |
Frozen Mouse Embryo | C.129S1-Il12rb1<tm1Jm>/J | $2595.00 |
Frozen Mouse Embryo | C.129S1-Il12rb1<tm1Jm>/J | $2595.00 |
Frozen Mouse Embryo | C.129S1-Il12rb1<tm1Jm>/J | $3373.50 |
Frozen Mouse Embryo | C.129S1-Il12rb1<tm1Jm>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.