B6.Cg-Kitl^Sl-18H/EiJ

Stock number: 002993
Product name: steel 18 Harwell
Research areas: Cancer Research, Dermatology Research, Developmental Biology Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research, Reproductive Biology Research

Development

The steel 18 Harwell mutation, then on a mixed C3H and 101 background, was imported in 1988 from Harwell into NCI, Frederick. There, Peter Donovan maintained it by backrossing to C3H/HeN and C3H/HeJ. In 1993 this mutation was imported from NCI to the laboratory of Eva Eicher at The Jackson Laboratory and the estimated backcross generation at that time was greater than N10. The Eicher laboratory then backcrossed this mutation onto a B6 background using both C57BL/6JEi and C57BL/6J.

Allele

Symbol: Kitl^Sl-18H
Name: steel 18 Harwell
MGI accession ID: MGI:1856366
Generation method: Radiation induced
Marker symbol: Kitl
Marker name: kit ligand
Marker synonyms: blz; DCUA; DFNA69; FPH2; FPHH; Gb; grizzle-belly; Kitlg; KL-1; Mgf; SCF; SF; SHEP7; Sl; SLF; Steel; Steel factor; stem cell factor; WS2F
Chromosome: 10
Strain of origin: (C3H/HeH x 101/H)F1
Molecular note: This allele comprises a deletion that is cytologically apparent, removing ~2.5% of Chr 10. Southern blot analysis and pulsed field gel electrophoresis showed that this deletion is greater than 810kb.