These Vcam1 knock-out mice exhibit a defect in placental development upon early embryonic death or heart abnormalities if surviving to later embryonic development. They are suitable for use in applications related to the study of placenta and heart dvelopment.
Dr. Mark A. Labow, Novartis Corporation
Mice homozygous for the Vcam1tm1Roml targeted mutation are not viable and exhibit either of two phenotypes. Approximately half the VCAM-deficient embryos die prior to embryonic day 11.5 exhibiting a severe defect in placental development in which the allantois failed to fuse with the chorion. The other half of the VCAM-deficient embryos survive to embryonic day 11.5-12.5 and display several heart abnormalities. Significant amounts of blood is found in the heart and pericardial space and embyros lack an epicardium.
The strain was developed by disruption of the murine Vcam gene by targeted homologous recombinationin ES cells and the introduction of the mutant ES cells into host blastocysts. The targeting vector introduced a frame shift and deletion mutation into the Vcam locus. In addition, a PGKneo resistance cassette was inserted within the coding region of the first Ig domain (exon 2). The construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+-derived W9.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6 mice.
|Allele Name||targeted mutation 1, Mark A Labow|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||VCAM-1 -|
|Gene Symbol and Name||Vcam1, vascular cell adhesion molecule 1|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|Molecular Note||A PGK-neomycin resistance expression cassette replaced a genomic fragment containing part of exon 2 and exons 3-5. These sequences encode the Ig domains.|
When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony. Mice homozygous for the mutation are not viable.
When using the VCAM-1 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #002992 in your Materials and Methods section.