These cΒ–STOP–lacZ mice express a transgene containing lacZ and a loxP flanked-STOP codon preventing lacZ expression.
David J Anderson, California Institute of Technology
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Reporter) |
This mutant, when crossed with a cre transgenic, will express lacZ in cells where cre is expressed to remove the STOP of translation section which lies between the 2 loxP sites. LacZ expression is restricted to neural and skeletal muscle tissue and heart by the chicken beta-actin promoter that is driving the reporter.
The cassette is "a loxP-STOP-of-translation-loxP-lacZ" combination. This is driven by a beta-actin promoter. The lacZ is expressed only in cells where the STOP element has been removed. This strain was generated on a (C57BL/6J x DBA/2)F1 genetic background.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
---|---|
Site of Expression | when crossed with a Cre recombinase expressing strain, lacZ will express in cells where cre is expressed; limited to neural tissue, skeletal muscle, and heart |
Allele Name | transgene insertion 32, David J Anderson |
---|---|
Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | cAct-XstopXlacZ; cBeta-STOP-lacZ; N-lacZ; Tg(xstpx-lacZ)32And |
Gene Symbol and Name | Tg(CAG-lacZ)32And, transgene insertion 32, David J Anderson |
Gene Synonym(s) | |
Promoter | CAG, CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | when crossed with a Cre recombinase expressing strain, lacZ will express in cells where cre is expressed; limited to neural tissue, skeletal muscle, and heart |
Strain of Origin | Not Specified |
Chromosome | UN |
General Note | Line 36 was also produced. The chicken beta actin promoter drives lacZ expression in neural tissue, skeletal muscle tissue, and heart. In conjunction with a cre transgene, transgenic mice express lacZ in cells where Cre is expressed. |
Molecular Note | The transgene is loxP-STOP-of-translation-loxP-lacZ driven by the chicken beta-actin promoter. |
When maintaining a live colony, these mice were bred as homozygotes. Coat color expected from breeding:Black
When using the cΒ–STOP–lacZ mouse strain in a publication, please cite the originating article(s) and include JAX stock #002982 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Carrier or non-carrier for Tg(xstpx-lacZ)32And |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.