B6.Cg-Tg(CAG-lacZ)32And/J

Stock number: 002982
Product name: cΒ–STOP–lacZ
Research areas: Neurobiology Research, Research Tools

Description

These cΒ–STOP–lacZ mice express a transgene containing lacZ and a loxP flanked-STOP codon preventing lacZ expression.

Detailed description

This mutant, when crossed with a cre transgenic, will express lacZ in cells where cre is expressed to remove the STOP of translation section which lies between the 2 loxP sites. LacZ expression is restricted to neural and skeletal muscle tissue and heart by the chicken beta-actin promoter that is driving the reporter.

Development

The cassette is "a loxP-STOP-of-translation-loxP-lacZ" combination. This is driven by a beta-actin promoter. The lacZ is expressed only in cells where the STOP element has been removed. This strain was generated on a (C57BL/6J x DBA/2)F1 genetic background.

Allele

Symbol: Tg(CAG-lacZ)32And
Name: transgene insertion 32, David J Anderson
MGI accession ID: MGI:3054996
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(CAG-lacZ)32And
Marker name: transgene insertion 32, David J Anderson
Chromosome: UN
Strain of origin: Not Specified
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: when crossed with a Cre recombinase expressing strain, lacZ will express in cells where cre is expressed; limited to neural tissue, skeletal muscle, and heart
Molecular note: The transgene is loxP-STOP-of-translation-loxP-lacZ driven by the chicken beta-actin promoter.
General note: Line 36 was also produced.

The chicken beta actin promoter drives lacZ expression in neural tissue, skeletal muscle tissue, and heart. In conjunction with a cre transgene, transgenic mice express lacZ in cells where Cre is expressed.