C57BL/6-Tg(Cd152Ig)1Jbs/J

Stock number: 002980
Product name: CTLA-4Ig
Research areas: Cancer Research, Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research

Description

These CTLA-4Ig mice express a transgene containing a mouse Ctla4/Ighg2a (formerly Igh-1a) fusion cDNA under control of the human keratin 14 enhancer/promoter.

Detailed description

A soluble form of the CD152 antigen (CTLA4Ig)is expressed under the control of the K14 promoter that is primarily active in the skin, but also thymus and tongue. The animal produces blood circulating levels of CTLA4Ig that are easily detectable by ELISA or functional assays.

Allele

Symbol: Tg(Cd152Ig)1Jbs
Name: transgene insertion 1, Jeffrey Bluestone
MGI accession ID: MGI:3054966
Generation method: Transgenic
Attributes: Inserted expressed sequence
Synonyms: CTLA4Ig; CTLA-4Ig; CTLA4-Ig; sCTLA4; Tg(Cd152Ig)
Marker symbol: Tg(Cd152Ig)1Jbs
Marker name: transgene insertion 1, Jeffrey Bluestone
Marker synonyms: CTLA4Ig; CTLA-4Ig; CTLA4-Ig; sCTLA4; Tg(Cd152Ig)
Chromosome: UN
Strain of origin: C3H
Expressed genes: Ighg2a,immunoglobulin heavy constant gamma 2A,mouse, laboratory; Ctla4,cytotoxic T-lymphocyte-associated protein 4,mouse, laboratory
Molecular note: The transgene contains a mouse Ctla4/Igh-1a fusion cDNA from which a soluble form of the CD152 antigen (CTLA4Ig) is expressed under control of the human keratin 14 (KRT14) enhancer/promoter, which directs expression to basal squamous epithelial cells primarily of the skin, thymus and tongue. Expression is enhanced by inclusion in the construct of most of the human growth hormone gene to provide intronic, 3' non-coding and polyadenylation sequences. Transgenic mice show blood circulating blood levels of CTLA4Ig that are easily detectable by ELISA or functional assays.