Homozygous SOD1m1BCM knock-out mice are characterized by abnormal retinal and cochlear ganglions, and they have age-related defects in hearing, vasculature, and muscle. These mice may be useful in studying retinal dysfunction, autoimmunity, female reproductive development/function, glaucoma, hearing loss, Alzheimer's Disease, familial amyotrophic lateral sclerosis (ALS), and other age-related disorders.
Dr. Russell Lebovitz, SUMA Partners
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Sod1 | superoxide dismutase 1, soluble |
The SOD1m1BCM knock-out allele disrupts exons 1 and 2 of the Cu,Zn-superoxide dismutase-1 gene (Sod1). No protein expression from the targeted locus is detected in brain tissues. Heterozygous mice are viable and fertile. While homozygous males reproduce normally, homozygous females are infertile. Homozygous mice have abnormalities of the retinal ganglion, cochlear ganglion, and female reproductive development/function. Homozygous mice also exhibit age-related deafness, accelerated vascular aging and age-related skeletal muscle defects. These mutant mice may be useful in studying retinal dysfunction, autoimmunity, glaucoma, hearing loss, Alzheimer's Disease, familial amyotrophic lateral sclerosis (ALS), and other age-related disorders.
A targeting construct was designed to delete exons 1 and 2 of the Cu,Zn-superoxide dismutase-1 gene (Sod1) and replace those exons with a PGK-HPRT expression cassette. This construct was electroporated into 129S7/SvEvBrd-Hprtb-m2-derived AB2.1 embryonic stem (ES) cells. The resulting mutant mice (SOD1m1BCM) were on a mixed C57BL/6;129SvEv genetic background when they arrived at The Jackson Laboratory Repository in 1997. Upon arrival, mutant mice were bred together, and then to B6129SF1/J (Stock No. 101043) for one generation. After this, heterozygous mice were bred together to maintain the colony.
Allele Name | targeted mutation 1, Russell M Lebovitz |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Cu,Zn-SOD-; CuZnSOD-; SOD1-; SOD1m1BCM |
Gene Symbol and Name | Sod1, superoxide dismutase 1, soluble |
Gene Synonym(s) | |
Strain of Origin | 129S7/SvEvBrd-Hprtb-m2 |
Chromosome | 16 |
Molecular Note | Replacement of exons 1 and 2 with a PGK-hprt expression cassette. Protein was not detected in brain of homozygous mutant mice. |
Mutations Made By | Dr. Russell Lebovitz, SUMA Partners |
When maintaining a live colony, heterozygous mice are bred together, or heterozygous females are bred to homozygous males. Female homozygous mice are infertile, though male homozygous mice reproduce normally. Average litter size is observed. Expected coat color from breeding is white bellied agouti or black.
When using the Sod1 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #002972 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Sod1<tm1Leb> |
Frozen Mouse Embryo | B6;129S-Sod1<tm1Leb>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129S-Sod1<tm1Leb>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129S-Sod1<tm1Leb>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6;129S-Sod1<tm1Leb>/J Frozen Embryos | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.