These Itgae knock-out mice exhibit reduced numbers of intestinal and vaginal intraepithelial lymphocytes. They are suitable for use in applications for studying the role of αεβ7 and of αεβ7-expressing mucosal T lymphocytes in vivo.
Dr. Christina M. Parker, Dana-Farber Cancer Institute
Mice that are homozygous for the Itgaetm1Cmp targeted mutation are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. No Itgae expression is detected in intraepithelial lymphocytes by FACS analysis or on TGF-beta1-treated splenocytes by immunoprecipitation. Homozygous null mice exhibit reduced numbers of intestinal intraepithelial lymphocytes and lamina propria lymphocytes.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 10 of the Itgae gene. The construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into BALB/c blastocysts. The resulting chimeric animals were backcrossed to BALB/c mice.
|Allele Name||targeted mutation 1, Christina M Parker|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||alphaE-; CD103-|
|Gene Symbol and Name||Itgae, integrin alpha E, epithelial-associated|
|Strain of Origin||129S2/SvPas|
|Molecular Note||Replacement of exon 10 with a neomycin resistance gene. No Itgae expression is detected in intraepithelial lymphocytes (IEL) by FACS analysis.|
|Mutations Made By|| |
Dr. Christina Parker, Dana-Farber Cancer Institute
This strain originated on C;129S2 background and has been backcrossed to BALB/c for ten generations before being made homozygous.
When using the αε- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002964 in your Materials and Methods section.