C.129S2-Itgae^tm1Cmp/J

Stock number: 002964
Product name: α_ε-
Research areas: Cell Biology Research, Hematological Research, Research Tools

Description

These Itgae knock-out mice exhibit reduced numbers of intestinal and vaginal intraepithelial lymphocytes. They are suitable for use in applications for studying the role of α_εβ₇ and of α_εβ₇-expressing mucosal T lymphocytes in vivo.

Detailed description

Mice that are homozygous for the Itgae^tm1Cmp targeted mutation are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. No Itgae expression is detected in intraepithelial lymphocytes by FACS analysis or on TGF-beta1-treated splenocytes by immunoprecipitation. Homozygous null mice exhibit reduced numbers of intestinal intraepithelial lymphocytes and lamina propria lymphocytes.

Development

A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 10 of the Itgae gene. The construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into BALB/c blastocysts. The resulting chimeric animals were backcrossed to BALB/c mice.

Allele

Symbol: Itgae^tm1Cmp
Name: targeted mutation 1, Christina M Parker
MGI accession ID: MGI:2136817
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Itgae
Marker name: integrin alpha E, epithelial-associated
Chromosome: 11
Strain of origin: 129S2/SvPas
Molecular note: Replacement of exon 10 with a neomycin resistance gene. No Itgae expression is detected in intraepithelial lymphocytes (IEL) by FACS analysis.