These Pcsk2 knock-out mice exhibit impaired processing of three prohormones of the islets of Langerhans: proinsulin, proglucagon, and prosomatostatin. They are suitable for use in applications related to the study of the physiological roles of neuropeptides and peptide hormones.
Dr. Donald Steiner, University of Chicago
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Pcsk2 | proprotein convertase subtilisin/kexin type 2 |
This strain was developed in the laboratory of Dr. Donald Steiner, HHMI Research Laboratories, The University of Chicago. Exon 3 of the Pcsk2 gene was interrupted using a vector containing the neo resistance gene. This results in a truncated transcript that is unstable, isn't secreted, and is unable to process protein precursors. The 129X1/SvJ x 129S1/Sv-derived R1 ES cell line was used for the targeted mutagenesis
Allele Name | targeted mutation 1, Donald Steiner |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | PC2-; PC2KO; SPC2- |
Gene Symbol and Name | Pcsk2, proprotein convertase subtilisin/kexin type 2 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 2 |
General Note | Phenotypic Similarity to Human Syndrome: Pancreatic Neuroendocrine Tumors J:236511. |
Molecular Note | A neomycin resistance cassette was inserted into exon 3, which encodes the activation site at the junction of the propeptide and mature enzyme. RT-PCR experiments demonstrated that exon 3 was absent from transcripts produced from this allele, but Western blot analysis revealed the presence of a truncated protein of a size predicted by this mutation. However, no mature processed protein was detected and there was no evidence of enzyme activity in homozygous mice. |
Mutations Made By | Dr. Donald Steiner, University of Chicago |
This strain is currently being maintained heterozygous sibling matings. Homozygous breeder pairs have a low reproductive performance. Expected coat color from breeding:Black, White Bellied Agouti
When using the SPC2- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002963 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Pcsk2<tm1Dfs> |
Frozen Mouse Embryo | B6;129-Pcsk2<tm1Dfs>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Pcsk2<tm1Dfs>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Pcsk2<tm1Dfs>/J | $3373.50 |
Frozen Mouse Embryo | B6;129-Pcsk2<tm1Dfs>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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