Mice homozygous for the Cd1tm1Gru targeted mutation are deficient in both the Cd1d1 and Cd1d2 genes (CD1.1, CD1.2). Homozygous mutant mice lack the normal natural killer cell-like T cell subset. However, type 2 T helper cell (TH2) response is normal, demonstrating that this T cell subset is not required for TH2 response, as previously believed.
The targeting vector used deleted most of the Cd1d1 and Cd1d2 coding regions. The intergenic sequence was maintined.
|Allele Name||targeted mutation 1, Michael Grusby|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||CD1-; CD1d KO; Cd1d-; Cd1d1/Cd1d2tm1Gru; CD1tm1Gru|
|Gene Symbol and Name||Cd1, CD1 antigen complex|
|Strain of Origin||129S2/SvPas|
|Molecular Note||Both the Cd1d1 gene and the adjacent Cd1d2 gene were mutated in this allele. The coding sequence of Cd1d1 was replaced with a neomycin selection cassette and the coding sequence of Cd1d2 was deleted; however, the intergenic sequence was retained. Flow cytometric analysis on thymocytes derived from homozygous mice confirmed that no stable encoded protein was expressed on the cell surface.|
|Mutations Made By|| |
Dr. Michael Grusby, Harvard Medical School
This strain is maintained by breeding homozygous sibling matings. Expected coat color from breeding:Albino
When using the CD1- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002962 in your Materials and Methods section.