C;129S-Cd1^tm1Gru/J

Stock number: 002962
Product name: CD1^-
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

These Cd1 knock-out mice exhibit deficiency of both CD1.1 and CD1.2, and lack NK-like T cells.

Detailed description

Mice homozygous for the Cd1^tm1Gru targeted mutation are deficient in both the Cd1d1 and Cd1d2 genes (CD1.1, CD1.2). Homozygous mutant mice lack the normal natural killer cell-like T cell subset. However, type 2 T helper cell (TH2) response is normal, demonstrating that this T cell subset is not required for TH2 response, as previously believed.

Development

The targeting vector used deleted most of the Cd1d1 and Cd1d2 coding regions. The intergenic sequence was maintined.

Allele

Symbol: Cd1^tm1Gru
Name: targeted mutation 1, Michael Grusby
MGI accession ID: MGI:1857482
Generation method: Targeted
Attributes: Null/Knockout
Synonyms: CD1^-; CD1d KO; Cd1d^-; Cd1d1/Cd1d2^tm1Gru; CD1tm1Gru
Marker symbol: Cd1
Marker name: CD1 antigen complex
Chromosome: 3
Strain of origin: 129S2/SvPas
Molecular note: Both the Cd1d1 gene and the adjacent Cd1d2 gene were mutated in this allele. The coding sequence of Cd1d1 was replaced with a neomycin selection cassette and the coding sequence of Cd1d2 was deleted; however, the intergenic sequence was retained. Flow cytometric analysis on thymocytes derived from homozygous mice confirmed that no stable encoded protein was expressed on the cell surface.