B6NTac.Cg-H2-Ab1^tm1Gru Tg(Ab1TL)1Gru/J

Stock number: 002956
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

These mice express a transgene containing only truncated MHC class II A beta molecules lacking the 13 membrane distal residues.

Detailed description

Hemizygous transgenic mice expressing only truncated class II A beta molecules lacking the 13 membrane distal residues were generated to address the role of class II cytoplasmic domains in physiological, non-transformed APC and in vivo immune responses. CD4⁺ T cell development and immune responses to conventional protein antigens, parasitic infections and skin grafts were indistinguishable between control and transgenic mice in vivo. However, in vitro, APC from transgenic mice poorly stimulate T cell hybridomas and wild-type in vivo-primed T cells. Class II-mediated induction of B7-1 expression and homotypic aggregation were not diminished in transgenic B cells, suggesting that both cAMP and tyrosine kinase signaling pathways remain intact despite truncation of the A beta cytoplasmic domain.

Allele

Symbol: Tg(Ab1TL)1Gru
Name: transgene insertion 1, Michael J Grusby
MGI accession ID: MGI:3054941
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Ab1TL)1Gru
Marker name: transgene insertion 1, Michael J Grusby
Chromosome: UN
Strain of origin: C57BL/6
Expressed genes: H2-Ab1,histocompatibility 2, class II antigen A, beta 1,mouse, laboratory
Molecular note: This beta chain transgene expresses a truncated class II A beta molecules that lack the 13 membrane distal residues. Transgene expression was detected on B cells and stimulated macrophages, with the expression on B cells being about a log unit higher than endogenous expression. Immunoprecipitation of B cell lysates confirmed a truncated I-Abeta chain was being expressed.

Allele

Symbol: H2-Ab1^tm1Gru
Name: targeted mutation 1, Michael J Grusby
MGI accession ID: MGI:2158420
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: H2-Ab1
Marker name: histocompatibility 2, class II antigen A, beta 1
Chromosome: 17
Strain of origin: 129S2/SvPas
Molecular note: A neomycin selection cassette was inserted into exon 2. In addition, the ES cell line used was derived from the 129S2/SvPas strain, which carries a deletion in the promoter region of H2-Ea. Consequently, these MHC class II molecule-deficient mice lacked cell surface expression of both class II-A and class II-E MHC proteins.