Mice hemizygous or homozygous for the ROSA26 retroviral insertion display no distinguishing phenotype. lacZ is expressed in all tissues of the developing embryo and in most tissues of the adult mouse.
Dr. Jennifer Payne, Harvard Medical School
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Gene trapped (Not Applicable) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
Mice hemizygous or homozygous for the ROSA26 retroviral insertion display no distinguishing phenotype. lacZ is expressed in all tissues of the developing embryo and in most tissues of the adult mouse.
This strain was developed in the laboratory of Dr. Philippe Soriano at the Fred Hutchinson Cancer Center, Seattle, WA. This mutant was made by a retroviral insertion of the beta-galactosidase gene (and accompanying splice acceptor and neo sequences) into ES cells. The construct was inserted in reverse orientation in the retroviral vector pGen-. The BALB/cJ strain was generated by backcrossing mice carrying the mutation 5 times to BALB/cJ inbred mice.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | All tissues of the developing embryo and in most tissues of the adult mouse. |
Allele Name | gene trap ROSA 26, Philippe Soriano |
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Allele Type | Gene trapped (Not Applicable) |
Allele Synonym(s) | gtrosa26; R26tg; R26R3; ROSA26; Rosa26lacZ; ROSA26-lacZ; TgR(ROSA26)26Sor |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | All tissues of the developing embryo and in most tissues of the adult mouse. |
Strain of Origin | 129S7/SvEvBrd-Hprt+ |
Chromosome | 6 |
General Note | ES cell line = AB1 or AB2.1. No overt phenotypes in heterozygous or homozygous animals. The trapped gene appears to be ubiquitously expressed during embryonic development and in all hematopoietic cells. |
Molecular Note | Random insertion of retroviral gene trap vector ROSA, Reverse Orientation with Splice Acceptor, which contains the beta-galactosidase gene and accompanying splice acceptor and neo sequences. The construct was inserted in reverse orientation in the retroviral vector pGen-. The transgene is controlled by an unknown endogenous promoter. lacZ is expressed in all tissues of the developing embryo and in most tissues of the adult transgenic mouse. |
Mutations Made By | Dr. Philippe Soriano, Mount Sinai School of Medicine |
This strain is maintained by breeding hemizygous female mice with hemizygous males. Homozygous mutant mice will breed, but their reproductive performance may be decreased. Expected coat color from breeding:Albino
When using the ROSA26-lacZ mouse strain in a publication, please cite the originating article(s) and include JAX stock #002955 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wild-type for Gt(ROSA)26Sor |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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