C.129S7-Gt(ROSA)26Sor/J

Stock number: 002955
Product name: ROSA26-lacZ
Research areas: Research Tools

Detailed description

Mice hemizygous or homozygous for the ROSA26 retroviral insertion display no distinguishing phenotype. lacZ is expressed in all tissues of the developing embryo and in most tissues of the adult mouse.

Development

This strain was developed in the laboratory of Dr. Philippe Soriano at the Fred Hutchinson Cancer Center, Seattle, WA. This mutant was made by a retroviral insertion of the beta-galactosidase gene (and accompanying splice acceptor and neo sequences) into ES cells. The construct was inserted in reverse orientation in the retroviral vector pGen-. The BALB/cJ strain was generated by backcrossing mice carrying the mutation 5 times to BALB/cJ inbred mice.

Allele

Symbol: Gt(ROSA)26Sor
Name: gene trap ROSA 26, Philippe Soriano
MGI accession ID: MGI:1890203
Generation method: Gene trapped
Attributes: Not Applicable
Marker symbol: Gt(ROSA)26Sor
Marker name: gene trap ROSA 26, Philippe Soriano
Chromosome: 6
Strain of origin: 129S7/SvEvBrd-Hprt⁺
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: All tissues of the developing embryo and in most tissues of the adult mouse.
Molecular note: Random insertion of retroviral gene trap vector ROSA, Reverse Orientation with Splice Acceptor, which contains the beta-galactosidase gene and accompanying splice acceptor and neo sequences. The construct was inserted in reverse orientation in the retroviral vector pGen-. The transgene is controlled by an unknown endogenous promoter. lacZ is expressed in all tissues of the developing embryo and in most tissues of the adult transgenic mouse.
General note: ES cell line = AB1 or AB2.1. No overt phenotypes in heterozygous or homozygous animals. The trapped gene appears to be ubiquitously expressed during embryonic development and in all hematopoietic cells.