Il2ra knock-out mice may be useful for studying lymphoproliferative disorder, hemolytic anemia and inflammatory bowel disease. This strain also is available on the BALB background (Stock No. 036023) and mixed 129 and C57BL/6 congenic background (Stock No. 002462).
IMR Colony, The Jackson Laboratory
Young mice homozygous for the Il2ratm1Dw knock-out mutation have phenotypically normal development of T and B cells. Adults develop massive enlargement of peripheral lymphoid organs associated with polyclonal T and B cell expansion resulting in autoimmune disorders, including hemolytic anemia and inflammatory bowel disease beginning at approximately 9 weeks of age. The knocked-out gene is also known as p55 gene chain, Ly43, or CD25.
This strain was developed in the laboratory of Dr. Dennis Willerford, Children's Hospital, Boston. A neomycin resistance cassette replaced a 5.5 kb segment of the gene, containing exons 2 and 3, which encode for the binding site of the protein. Correctly targeted, transfected J1 ES cells were injected into C57BL/6 blastocysts. The strain has been backcrossed for 7 generations to C57BL/6J before being maintained by inbreeding.
|Allele Name||targeted mutation 1, Dennis M Willerford|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||CD25-; CD25KO; IL-2Ralpha-; IL-2Ralpha KO; IL2Ralpha-|
|Gene Symbol and Name||Il2ra, interleukin 2 receptor, alpha chain|
|Strain of Origin||129S4/SvJae|
|Molecular Note||A neomycin resistance cassette replaced a 5.5 kb segment of the gene, containing exons 2 and 3, which encode for the binding site of the protein.|
|Mutations Made By|| |
Dr. Dennis Willerford, Children's Hospital
When maintaining a live colony, heterozygous mice may be bred as together. Such matings may produce less homozygotes than Mendelian expectations. Homozygous females may not be healthy enough to breed. The expected coat color from breeding is black.
When using the IL-2Rα- mouse strain in a publication, please cite the originating article(s) and include JAX stock #002952 in your Materials and Methods section.