C.B6-Klra8^Cmv1-r/UwaJ

Stock number: 002936
Product name: Cmv-1^l
Research areas: Research Tools, Virology Research

Description

These BALB/c congenic mice carry the Klra8^Cmv1-r allele from C57BL/6 that confers resistance to lethal infection with murine cytomegalovirus (MCMV).

Detailed description

This BALB/c congenic carries C57BL/6 alleles for loci in the natural killer cell gene complex (NKC) on mouse chromosome 6. The region contains Klra (formerly Ly49), Klrb1 (formerly Ly55) and Klra8. This strain is useful in tumor models involving NK cell regulation and the expression of NK1.1 on BALB/c background. It plays a role in elucidating the contribution of the NKC region in controlling infectious agents.

Development

Made from donor strain C57BL/6 and recipient strain BALB/c. At each generation mice used in next backcross were screened for Cmvl^r allele. After 10 generations of backcrossing, brother x sister matings were initiated to generate homozygous individuals. Representative mice from the colony maintained by Scalzo at University of Western Australia were sent to his collaborator Yokoyama at Washington University in St. Louis. The Yokoyama colony is the source of TJL mice.

Allele

Symbol: Klra8^Cmv1-r
Name: cytomegalovirus resistance 1, resistant
MGI accession ID: MGI:2387148
Generation method: Not Applicable
Marker symbol: Klra8
Marker name: killer cell lectin-like receptor, subfamily A, member 8
Chromosome: 6
Strain of origin: C57BL/6
Molecular note: Mapping experiments confirmed that the C57BL/6 strain contained the Klra8 gene, and expression of Klra8 correlated with the resistant phenotype.